PMID: 8597578Feb 8, 1996Paper

Evidence for a proprotein intermediate during maturation of type II antifreeze protein in sea raven, Hemitripterus americanus

Biochimica Et Biophysica Acta
B P DunckerP L Davies

Abstract

The circulating Type II antifreeze protein (AFP) in sea raven is 129 amino acids (aa) long (14 kDa) and is derived from an initial 163 aa translation product that is synthesised in the liver. Signal peptide cleavage algorithms, as well as transgenic expression studies in fall armyworm cells, predict the formation of a 146 aa (16 kDa) proprotein intermediate. A protein of this size that cross-reacted with anti-sea raven AFP antibody was detected in sea raven serum using phosphate/urea SDS-PAGE, and was purified by size-exclusion chromatography and reverse-phase HPLC. N-terminal sequencing and mass spectrometry identified the protein as the predicted proAFP, and immunoblotting suggested that it is the predominant form present in liver. These results are consistent with production and storage of a proAFP intermediate in the liver, and its subsequent processing to mature AFP during or soon after its release into the circulation.

References

May 29, 1992·Biochemical and Biophysical Research Communications·K V EwartG L Fletcher
Dec 18, 1991·European Journal of Biochemistry·A Chakrabartty, C L Hew
Oct 31, 1991·Biochemical and Biophysical Research Communications·B RubinskyG L Fletcher
Jun 11, 1986·Nucleic Acids Research·G von Heijne
Mar 1, 1995·Protein Science : a Publication of the Protein Society·F D SönnichsenP L Davies
Sep 30, 1994·Biochemical and Biophysical Research Communications·B P DunckerP L Davies
Apr 1, 1994·Cryobiology·S R PayneG C Upreti
Oct 7, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ronen SadehAaron Ciechanover

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Citations

Jul 21, 2004·Biochimica Et Biophysica Acta·Robert P Evans, Garth L Fletcher
Dec 7, 2005·Protein Expression and Purification·Andrew J ScotterDavid R Rose

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