Evidence for a role of the sarcoplasmic/endoplasmic reticulum Ca(2+)-ATPase in thapsigargin and Bcl-2 induced changes in Xenopus laevis oocyte maturation

Oncogene
E M Kobrinsky, M A Kirchberger

Abstract

Thapsigargin (Tg), a selective inhibitor of sarcoplasmic/endoplasmic reticulum Ca(2+)-ATPase (SERCA), causes depletion of intracellular Ca(2+) stores, hence activation of capacitative Ca(2+) entry (CCE). Incubation of Xenopus laevis oocytes with Tg resulted in an increased rate of progesterone-induced meiotic maturation. Non-mitochondrial (45)Ca(2+) uptake by SERCA-containing microsomes prepared from control wild-type oocytes microinjected with sterile water was inhibited essentially 100% by Tg. However, overexpression of Bcl-2, an oncogene known to protect against Tg-induced apoptosis in certain cell types, resulted in only 40% inhibition of microsomal (45)Ca(2+) uptake by Tg while non-inhibited (45)Ca(2+) uptake remained unchanged. Moreover Bcl-2 overexpression also protected against inhibition of CCE. I(Cl(Ca)) was similar in Bcl-2-overexpressing and control oocytes when intracellular Ca(2+) store depletion was induced by microinjection of inositol 1,4,5-trisphosphate (InsP(3)) and other means and when CCE was induced by means independent of SERCA inhibition. Our data indicate that Bcl-2 affects neither the InsP(3) receptor nor Ca(2+) entry itself. At the end of a 24-h period after progesterone addition to the medium, only 2...Continue Reading

References

Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·O ThastrupA P Dawson
Apr 1, 1989·The Journal of Experimental Zoology·J P VilainM Moreau
Jan 1, 1995·Pediatric Radiology·W S KimM C Han
Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·M LamC W Distelhorst
Aug 1, 1994·The American Journal of Physiology·A MattiazziD M Bers
Dec 15, 1995·The Journal of Biological Chemistry·L Stehno-BittelD E Clapham
Mar 1, 1996·Molecular and Cellular Biology·J J HunterT G Parslow
Sep 23, 1997·The Journal of Cell Biology·H HeC W Distelhorst
Apr 8, 1998·Current Opinion in Genetics & Development·K Newton, A Strasser
Oct 27, 1998·Oncogene·T H KuoW Tsang
Oct 28, 1998·Physiological Reviews·H K Simmerman, L R Jones
Oct 12, 1999·The American Journal of Physiology·E KobrinskyA R Marks
Dec 22, 1999·Archives of Biochemistry and Biophysics·J MahaneyT Muchnok
Mar 31, 2000·Surgical Endoscopy·G C VitaleG M Larson
Apr 15, 2000·The Journal of Biological Chemistry·Y FurukawaM Matsuda
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·R Foyouzi-YoussefiK H Krause
Aug 23, 2000·The Journal of Cell Biology·K NakamuraM Michalak
Sep 16, 2000·Biochemical Society Symposium·G Kroemer
May 1, 1982·Applied Biochemistry and Biotechnology·M KitY Matsui

❮ Previous
Next ❯

Citations

Apr 29, 2009·Zygote : the Biology of Gametes and Early Embryos·Inés RamosSilvia N Fernández
Sep 8, 2012·Cellular and Molecular Life Sciences : CMLS·Giovanni MonacoGeert Bultynck
Jan 31, 2013·Biochimica Et Biophysica Acta·Benjamin BonneauGermain Gillet
Dec 7, 2005·Developmental Biology·Wassim El-JouniKhaled Machaca
Oct 16, 2008·World Journal of Gastroenterology : WJG·Pei-Ming SongXian Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Biochemistry Research International
Mariusz K JaglarzJacek Z Kubiak
Zygote : the Biology of Gametes and Early Embryos
I RamosS Fernández
Biochemical and Biophysical Research Communications
Giovanni MonacoGeert Bultynck
© 2021 Meta ULC. All rights reserved