Evidence for a role of the ubiquitin-proteasome pathway in pancreatic islets

Diabetes
María D López-AvalosG C Weir

Abstract

The ubiquitin-proteasome pathway is crucial for protein turnover. Part of the pathway involves deubiquitination, which is carried out by cystein proteases known as ubiquitin COOH-terminal hydrolases. The isoform Uch-L1 was found to be present in large amounts in rat islets by immunostaining, Western blot analysis, and RT-PCR. Culturing islets in high glucose concentrations (16.7 mmol/l) for 24 h led to decreased gene expression. Exposure to chronic hyperglycemia following 90% partial pancreatectomy also led to reduced Uch-L1 expression. Expression of other members of the ubiquitin-proteasome pathway studied after culturing islets at high glucose concentrations revealed little change except for modest declines in parkin, human ubiquitin-conjugating enzyme 5 (UbcH5), and beta-TRCP (transducin repeat-containing protein). With the pancreatectomy model, expression of polyubiquitin-B and c-Cbl were increased and E6-associated protein was reduced. Further insight about the proteasome pathway was obtained with the proteasome inhibitor lactacystin, which in short-term 2-h experiments enhanced glucose-induced insulin secretion. An important role for the ubiquitin-proteasome pathways in beta-cells is suggested by the findings that changes...Continue Reading

References

Feb 1, 1985·Histopathology·J RodeR J Thompson
Nov 14, 1983·Brain Research·R J ThompsonJ Rode
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·M ScheffnerP M Howley
Feb 3, 1998·Molecular and Cellular Biology·Z LuD A Foster
Oct 6, 1998·Annual Review of Biochemistry·A Hershko, A Ciechanover
Oct 17, 1998·Nature·E LeroyM H Polymeropoulos
Oct 28, 1998·Trends in Cell Biology·D H Lee, A L Goldberg
Jan 9, 1999·Trends in Neurosciences·A Alves-RodriguesM E Figueiredo-Pereira
May 25, 1999·Cell·J D Laney, M Hochstrasser
Jul 14, 2000·American Journal of Physiology. Endocrinology and Metabolism·Y Q LiuJ L Leahy
Jul 19, 2000·Biochimica Et Biophysica Acta·F CheclerP Marambaud
Jul 25, 2000·Seminars in Cell & Developmental Biology·K D Wilkinson
Mar 27, 2001·Nature Reviews. Molecular Cell Biology·A M Weissman
Apr 3, 2001·Nature Reviews. Molecular Cell Biology·C B Thien, W Y Langdon
Jul 14, 2001·Science·C Haass, P J Kahle
Aug 28, 2001·Diabetes·S K SreenanK S Polonsky
Oct 25, 2001·Brain Research. Developmental Brain Research·D P HuynhS M Pulst
Jul 10, 2003·Molecular and Cellular Endocrinology·Tomo SaricKresimir Pavelic
Aug 9, 2003·Upsala Journal of Medical Sciences·Johnny SternesjöStellan Sandler
Aug 5, 2004·The Journal of Clinical Endocrinology and Metabolism·Rebecca L HullSteven E Kahn
Feb 12, 2005·The Journal of Biological Chemistry·Kajorn KitiphongspattanaH Rex Gaskins

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Citations

Sep 21, 2012·Current Diabetes Reports·Yoon Shin ChoChu Won Nho
Jan 26, 2013·DNA and Cell Biology·Shiying ShaoXuefeng Yu
Apr 17, 2012·International Journal of Endocrinology·Szu-Tah ChenBrend Ray-Sea Hsu
Nov 26, 2008·BMC Biochemistry·Simon S Wing
Mar 25, 2015·The Journal of Surgical Research·Monica P RodriguezMelina R Kibbe
Feb 2, 2015·Journal of Proteomics·B BrackevaG A Martens
May 23, 2015·Trends in Molecular Medicine·Abhisek MukherjeeClaudio Soto
Aug 12, 2014·Biochemical and Biophysical Research Communications·Kazuo HaraTakashi Kadowaki
Jun 25, 2015·PloS One·Hanneke HoelenEmmanuel J H J Wiertz
Sep 25, 2008·American Journal of Physiology. Endocrinology and Metabolism·Taila HartleyAllen Volchuk
Feb 25, 2010·Endocrine Journal·Hirotaka Watada
Apr 21, 2020·Free Radical Research·Takujiro Homma, Junichi Fujii
Sep 12, 2019·American Journal of Physiology. Renal Physiology·Hua SuChun Zhang
Jul 9, 2014·The Journal of Biological Chemistry·Sushant BhatnagarAlan D Attie
Nov 5, 2013·The Journal of Biological Chemistry·Anke Hofmeister-BrixSimone Baltrusch

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