Abstract
RGS proteins (regulators of G protein signalling) negatively regulate G protein function as GTPase-activating proteins (GAP) for G protein alpha-subunits. The existence of mRNAs of different size for some of the RGS proteins, e.g. RGS3, suggests that these proteins may exist in isoforms due to alternative splicing. We therefore investigated RGS3 mRNA and protein expression in different human tissues. Ribonuclease protection assays and Northern blot analysis showed two specific mRNAs for RGS3 (RGS3L, RGS3S) in human myocardium, suggesting an additional, N-terminally truncated form of approximately 168 aa. When expressed as a recombinant protein RGS3S was recognized at approximately 23 kDa by an antipeptide antiserum originally raised against an RGS2 sequence. In membranes of human tissues this antiserum detected specific signals for RGS3L (approximately 70 kDa), RGS2 (approximately 30 kDa) and a 25-kDa protein, most likely RGS3S. Both RGS3S mRNA and the 25 kDa protein were abundant in human heart, whereas expression in liver, brain and myometrium was much weaker. To characterize RGS3S functionally, single turnover GTPase, adenylyl cyclase (AC) and phospholipase C (PLC) activities were determined. Both recombinant RGS3S and RGS16...Continue Reading
Citations
Oct 2, 2003·Journal of Molecular and Cellular Cardiology·Clemens MittmannThomas Eschenhagen
Feb 1, 2003·Pharmacology & Therapeutics·Thomas Wieland, Clemens Mittmann
Oct 1, 2010·American Journal of Physiology. Cell Physiology·Fang LiWenhui Hu
May 1, 2012·PloS One·Yonggang ZhangWenhui Hu
Feb 18, 2016·Digestive Diseases and Sciences·Yonggang ZhangWenhui Hu
Dec 1, 2009·Gene·Fang LiWenhui Hu
Jan 27, 2005·The FEBS Journal·Yanbin LiangDavid F Woodward
Apr 16, 2008·European Journal of Pharmacology·Mariëlle C Hendriks-BalkAstrid E Alewijnse
Mar 29, 2014·PLoS Genetics·Jared E DeckerJeremy F Taylor
Feb 16, 2007·Cellular Signalling·Andreas VogtThomas Wieland
May 27, 2005·The Journal of Biological Chemistry·Chris HagueJohn R Hepler
Mar 19, 2019·Endoscopic Ultrasound·Suhag PatelMohammad Al-Haddad
Oct 26, 2007·American Journal of Physiology. Cell Physiology·Wenhui HuKarnam S Murthy
Sep 18, 2020·The Biochemical Journal·Peter G ChildsMatthew J Dalby
Jun 18, 2019·BMJ Open Sport & Exercise Medicine·Katherine M EdenfieldJames R Clugston
Jul 3, 2008·Clinical Science·David M HarrisAndrea D Eckhart