PMID: 6166472May 15, 1981Paper

Evidence for a signal sequence at the N terminus of the common precursor to adrenocorticothrophin and beta-lipotropin in mouse pituitary cells

European Journal of Biochemistry
P PolicastroM Chrétien

Abstract

The precursor to corticotropin and beta-endorphin was synthesized in a reticulocyte cell-free system under the direction of mRNA from mouse AtT-20 pituitary tumor cells in the presence of [3H]proline, [3H]phenylalanine, [3H]leucine, [3H]valine, [3H]isoleucine or [35S]methionine. Automatic Edman degradation of the radioactive cell-free product showed the following N-terminal sequence: Pro-1, Met-2, Leu-11, Leu-12, Leu-13, Leu-15, Leu-16, Leu-17, Ile-21 and Val-23. The corticotropin-endorphin precursor was also labeled in AtT-20 cells with [3H]valine, [3H]leucine, [3H]tryptophan, [3H]serine, [35S]methionine or [35S]cysteine. Automatic Edman degradation of the radioactive intact cell form gave the following N-terminal sequence: Trp-1, Cys-2, Leu-3, Ser-5, Ser-6, Val-7, Cys-8, Leu-11, Leu-17, Leu-18 and tentatively Met-27. The sequence of the intact cell form from AtT-20 cells matches the sequence of the cell-free form of bovine pituitary precursor beginning at Trp-27, as determined by recombinant DNA technology [Nakanishi, S., Inoue, A., Kita, T., Nakamura, M., Chang, A. C. Y., Cohen, S. N., and Numa, S. (1979) Nature (Lond.) 278, 423-427]. The sequence of the mouse pituitary mRNA-directed cell-free translation product also matche...Continue Reading

References

Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J F HabenerJ T Potts
Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R E MainsN Ling
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J L Roberts, E Herbert
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·P CrineM Chrétien
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·Y P Loh
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R C Jackson, G Blobel
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Jul 1, 1981·Journal of Agricultural and Food Chemistry·W M Walter, G L Catignani

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