PMID: 6403993Mar 1, 1983Paper

Evidence for a trans-dominant regulator of purine nucleoside phosphorylase expression in rat hepatoma cells

Somatic Cell Genetics
P A HoffeeP Jargiello

Abstract

Purine nucleoside phosphorylase (PNP) levels are modulated during the growth cycle of rat hepatoma cells and increase two- to three-fold as cells go from early exponential growth phase to stationary growth phase. A mutant of these hepatoma cells has been isolated which is deficient in PNP activity. Quantitative immunoprecipitation tests indicate that the decrease in enzyme activity is due to a decrease in the number of PNP molecules. The low level of PNP enzyme produced by the mutant, however, is indistinguishable from the wild-type enzyme, suggesting that the mutant may be defective in the ability to modulate PNP levels. Fusion of the mutant cells to wild-type parental cells results in hybrids that express the mutant phenotype. Segregants that arise from the hybrids show chromosome loss and reexpression of the wild-type parental phenotype, the mutant parental phenotype, and a 2S wild-type phenotype. These indicate the following about the defect in modulation in the mutant PNP-100: (1) it is trans dominant to the wild-type; (2) its effect is negative; (3) some genomic element is required for its continued effect; and (4) it does not act by obliterating its functioning counterpart in hybrid cells.

References

Apr 1, 1979·Archives of Biochemistry and Biophysics·R A May, P Hoffee
Apr 1, 1978·The Journal of Clinical Investigation·E W GelfandI H Fox
Dec 1, 1978·Biochemical Genetics·T A BremnerR E Kouri
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D A CarsonJ E Seegmiller
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·B S MitchellW N Kelley
May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D A CarsonL Thompson
Apr 1, 1979·Archives of Biochemistry and Biophysics·D W Lincoln, P Hoffee
Jan 1, 1979·Journal of Supramolecular Structure·M Uziel, J Selkirk
Mar 1, 1977·Somatic Cell Genetics·M L GefterM D Scharff
May 1, 1971·Annals of Human Genetics·Y H EdwardsH Harris

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