Evidence for Acquisition in Nature of a Chromosomal 2,4-Dichlorophenoxyacetic Acid/(alpha)-Ketoglutarate Dioxygenase Gene by Different Burkholderia spp.

Applied and Environmental Microbiology
V G MathesonWilliam E Holben

Abstract

We characterized the gene required to initiate the degradation of 2,4-dichlorophenoxyacetic acid (2,4-D) by the soil bacterium Burkholderia sp. strain TFD6, which hybridized to the tfdA gene of the canonical 2,4-D catabolic plasmid pJP4 under low-stringency conditions. Cleavage of the ether bond of 2,4-D by cell extracts of TFD6 proceeded by an (alpha)-ketoglutarate-dependent reaction, characteristic of TfdA (F. Fukumori and R. P. Hausinger, J. Bacteriol. 175:2083-2086, 1993). The TFD6 tfdA gene was identified in a recombinant plasmid which complemented a tfdA transposon mutant of TFD6 created by chromosomal insertion of Tn5. The plasmid also expressed TfdA activity in Escherichia coli DH5(alpha), as evidenced by enzyme assays with cell extracts. Sequence analysis of the tfdA gene and flanking regions from strain TFD6 showed 99.5% similarity to a tfdA gene cloned from the chromosome of a different Burkholderia species (strain RASC) isolated from a widely separated geographical area. This chromosomal gene has 77.2% sequence identity to tfdA from plasmid pJP4 (Y. Suwa, W. E. Holben, and L. J. Forney, abstr. Q-403, in Abstracts of the 94th General Meeting of the American Society for Microbiology 1994.). The tfdA homologs cloned fr...Continue Reading

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Citations

Feb 7, 2003·Applied and Environmental Microbiology·Philip DennisRoberta Fulthorpe
Aug 23, 2006·Critical Reviews in Biotechnology·Meltem Urgun-DemirtasKrishna Pagilla
Aug 28, 2014·Critical Reviews in Microbiology·Baljinder Singh, Kashmir Singh
Oct 6, 1998·Applied and Environmental Microbiology·C McGowanJ M Tiedje
Apr 16, 1998·Journal of Bacteriology·M L de SouzaL P Wackett
Nov 18, 2018·The Science of the Total Environment·Balasubramanian RamakrishnanMallavarapu Megharaj
May 16, 2001·Biotechnology and Bioengineering·S A JackmanC J Knowles
Nov 1, 1996·Applied and Environmental Microbiology·J DunbarL J Forney
Apr 1, 1997·Applied and Environmental Microbiology·J DunbarL Forney

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