Jan 25, 2011

Evidence for altered Numb isoform levels in Alzheimer's disease patients and a triple transgenic mouse model

Journal of Alzheimer's Disease : JAD
Srinivasulu ChigurupatiSic L Chan

Abstract

The cell fate determinant Numb exists in four alternatively spliced variants that differ in the length of their PTB (phosphotyrosine-binding domain, either lacking or containing an 11 amino acid insertion) and PRR (proline-rich region, either lacking or containing a 48 amino acid insertion). We previously reported that Numb switches from isoforms containing the PTB insertion to isoforms lacking this insertion in neural cultures subjected to stress induced by trophic factor withdrawal. The switch in Numb isoforms enhances the generation of amyloid-β peptide (Aβ), the principle component of senile plaques in Alzheimer's disease (AD). Here we examine the expression of the Numb isoforms in brains from AD patients and triple transgenic (3xTg) AD mice. We found that levels of the Numb isoforms lacking the PTB insertion are significantly elevated in the parietal cortex but not in the cerebellum of AD patients when compared to control subjects. Levels of Numb isoforms lacking the PTB insertion were also elevated in the cortex but not cerebellum of 12 month-old 3xTg AD mice with Aβ deposits compared to younger 3xTg-AD mice and to non-transgenic mice. Exposure of cultured neurons to Aβ resulted in an increase in the levels of Numb isofor...Continue Reading

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Mentioned in this Paper

Familial Alzheimer Disease (FAD)
Pathogenic Aspects
Transfection
Cortex Bone Disorders
Adrenal Cortex Diseases
Pathogenesis
APP protein, human
Glial Fibrillary Acidic Protein
Presenilin-1
Neurons

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