Evidence for alternative quaternary structure in a bacterial Type III secretion system chaperone.

BMC Structural Biology
Michael L BartaBrian V Geisbrecht

Abstract

Type III secretion systems are a common virulence mechanism in many Gram-negative bacterial pathogens. These systems use a nanomachine resembling a molecular needle and syringe to provide an energized conduit for the translocation of effector proteins from the bacterial cytoplasm to the host cell cytoplasm for the benefit of the pathogen. Prior to translocation specialized chaperones maintain proper effector protein conformation. The class II chaperone, Invasion plasmid gene (Ipg) C, stabilizes two pore forming translocator proteins. IpgC exists as a functional dimer to facilitate the mutually exclusive binding of both translocators. In this study, we present the 3.3 A crystal structure of an amino-terminally truncated form (residues 10-155, denoted IpgC10-155) of the class II chaperone IpgC from Shigella flexneri. Our structure demonstrates an alternative quaternary arrangement to that previously described for a carboxy-terminally truncated variant of IpgC (IpgC1-151). Specifically, we observe a rotationally-symmetric "head-to- head" dimerization interface that is far more similar to that previously described for SycD from Yersinia enterocolitica than to IpgC1-151. The IpgC structure presented here displays major differences i...Continue Reading

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Citations

May 19, 2011·The Journal of Biological Chemistry·Reinhard Albrecht, Kornelius Zeth
Dec 13, 2012·The Journal of Biological Chemistry·Sunny K SinghEwan R G Main
Jan 28, 2012·Acta Crystallographica. Section D, Biological Crystallography·Cheng DongYu Hui Dong
Mar 16, 2013·Protein Science : a Publication of the Protein Society·Shyamal P ChoudhariWendy L Picking
Feb 12, 2014·Biochimica Et Biophysica Acta·Brianne J Burkinshaw, Natalie C J Strynadka
May 14, 2011·Structure·Thierry IzoréAndréa Dessen
Dec 21, 2016·Molecular Microbiology·Jessica A KleinLeigh A Knodler
May 18, 2021·Frontiers in Cellular and Infection Microbiology·Mariana L FerrariAnastasia D Gazi

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Methods Mentioned

BETA
gel filtration
gel-filtration
PCR
X-ray
electrophoresis
Size Exclusion Chromatography

Software Mentioned

CLUSTALW
PHASER
EBI Protein Interfaces Surfaces and Assemblies ( PISA )
HKL2000
phenix
ESPRIPT
RAMPAGE
PyMol
refine
Protein Interfaces , Surfaces , and Assemblies server ( PISA )

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