Evidence for an activation domain at the amino terminus of simian immunodeficiency virus Vpx.

Journal of Virology
Thomas GrambergNathaniel R Landau

Abstract

Vpx and Vpr are related lentiviral accessory proteins that enhance virus replication in macrophages and dendritic cells. Both proteins are packaged into virions and mediate their effects in the target cell through an interaction with an E3 ubiquitin ligase that contains DCAF1 and DDB1. When introduced into primary macrophages and dendritic cells in viruslike particles, Vpx can enhance the efficiency of a subsequent infection. Here, we confirm the ability of Vpx to enhance simian immunodeficiency virus (SIV) and human immunodeficiency virus type 1 (HIV-1) infection of macrophages up to 100-fold by using single-cycle reporter viruses and by pretreatment of the cells with Vpx-containing viruslike particles. Vpx was also active in differentiated THP-1 cells but not in other cell lines. Induction of an antiviral state in macrophages with type I interferon significantly magnified the effect of Vpx on HIV-1 infection, suggesting that Vpx helps the virus to overcome an inducible intracellular restriction. Quantitative PCR quantitation of SIV and HIV-1 reverse transcripts in newly infected macrophages showed that the block was at an early step in reverse transcription. In spite of its structural similarity, Vpr was inactive. This differ...Continue Reading

References

Apr 1, 1995·Journal of Virology·J S GibbsR C Desrosiers
Feb 10, 1998·Virology·M TristemD L Quicke
Aug 15, 2002·Bioinformatics·B Contreras-Moreira, P A Bates
Oct 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Carsten MünkNathaniel R Landau
Oct 7, 2003·Nature Medicine·Mariana MarinDavid Kabat
Sep 13, 2005·Microbes and Infection·Boonruang KhamsriAkio Adachi
Feb 7, 2006·Virology·Lisa A MahnkeLee Ratner
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Bärbel SchröfelbauerNathaniel R Landau
Jul 5, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kasia HreckaJacek Skowronski
Jul 17, 2007·PLoS Pathogens·Jean-Philippe BelzileEric A Cohen
Jan 18, 2008·Nature·Stuart J D NeilPaul D Bieniasz
May 3, 2008·PLoS Pathogens·Natalia SharovaMario Stevenson
Jul 10, 2009·Journal of Virology·Fransje A KoningMichael H Malim

❮ Previous
Next ❯

Citations

Dec 14, 2012·Nature·Francis AlonzoVictor J Torres
Sep 14, 2012·Nature Medicine·Hanna-Mari BaldaufOliver T Keppler
Sep 14, 2012·Journal of Virology·Henning HofmannNathaniel R Landau
Mar 19, 2013·Retrovirology·Thomas GrambergNathaniel R Landau
Dec 21, 2012·Retrovirology·Yong-Hui ZhengKenzo Tokunaga
Oct 25, 2012·Retrovirology·Sarah WelbournKlaus Strebel
Nov 9, 2012·Retrovirology·Tao ZhouYong-Hui Zheng
Nov 10, 2011·Viruses·Jörg Zielonka, Carsten Münk
Oct 16, 2012·Current Opinion in Virology·Bizhan Romani, Eric A Cohen
Jul 1, 2011·Veterinary Immunology and Immunopathology·Eric M Poeschla
Dec 17, 2015·Retrovirology·Sabine WittmannThomas Gramberg
Jan 25, 2011·Molecular Microbiology·Ashley L DumontVictor J Torres
Oct 12, 2013·The Journal of General Virology·Ariko MiyakeYasuyuki Miyazaki
Nov 20, 2013·Current Opinion in Virology·Klaus Strebel
Jun 24, 2014·Microbes and Infection·Masako NomaguchiAkio Adachi
May 2, 2014·Frontiers in Microbiology·Torsten SchallerCaroline Goujon
Dec 20, 2016·Nature Medicine·Constanze SchneiderJindrich Cinatl
Aug 30, 2013·Journal of Virology·Henning HofmannNathaniel R Landau
Aug 29, 2012·Nature Reviews. Microbiology·Diana AyindeOlivier Schwartz
Sep 25, 2019·Nature Microbiology·Ramona BusingerMichael Schindler
Feb 24, 2017·Proceedings of the National Academy of Sciences of the United States of America·Hanna-Mari BaldaufOliver T Keppler
Jun 13, 2018·Nature Microbiology·Ghina ChouguiFlorence Margottin-Goguet
Oct 12, 2018·Proceedings of the National Academy of Sciences of the United States of America·Kirsten M KnechtYong Xiong
Apr 4, 2021·Viruses·Janina Deutschmann, Thomas Gramberg
Oct 27, 2021·PLoS Pathogens·Michaël M MartinFlorence Margottin-Goguet

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.