PMID: 6938981Dec 1, 1980

Evidence for an alternative nitrogen fixation system in Azotobacter vinelandii

Proceedings of the National Academy of Sciences of the United States of America
P E BishopD R Hetherington

Abstract

Two Azotobacter vinelandii strains capable of growing on N2(Nif+) were isolated from two different mutant strains that lacked dinitrogenase activity (Nif-). Extracts of N2-grown cells of the two Nif+ strains lacked significant amounts of the "conventional" dinitrogenase protein subunits, as determined by two-dimensional gel electrophoresis. Instead, the extracts contained at least four new proteins that appeared to be ammonia-repressible (i.e., they were not detected in extracts of ammonia-grown cells). Based on the results of genetic backcrosses, the two Nif+ strains were shown to be pseudorevertants. Both Nif+ pseudorevertant strains were able to grow in N-free media lacking molybdenum but containing tungsten (conditions that prevented growth of the wild-type strain). The four new proteins were observed in extracts of N2-fixing cells of the Nif+ pseudorevertants regardless of whether the cells were grown in the presence of molybdenum-starved wild-type A. vinelandii cells grown under N2-fixing conditions. Under conditions of molybdenum deprivation, Nif- mutant strains of several different phenotypic classes underwent phenotypic reversal to Nif+, as shown by their ability to incorporate 15N2 and to grow in N-free media. These r...Continue Reading

References

Jul 1, 1991·Molecular & General Genetics : MGG·F Luque, R N Pau
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D R Dean, K E Brigle
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K E BrigleW E Newton
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J T BolinN H Xuong
Jan 15, 2008·Applied and Environmental Microbiology·J P BellengerA M L Kraepiel
Jan 5, 2005·Journal of Bacteriology·Luis M Rubio, P W Ludden
Feb 1, 1987·Journal of Bacteriology·W H BingleW J Page
May 30, 2009·Proceedings of the National Academy of Sciences of the United States of America·Chi Chung LeeMarkus W Ribbe
May 1, 1989·Trends in Biochemical Sciences·R N Pau
Jan 11, 2016·World Journal of Microbiology & Biotechnology·Jesse Noar, José M Bruno-Bárcena
Sep 11, 2012·Journal of Basic Microbiology·Susanne HerterFrieder Schauer
Mar 2, 1991·Analytical Biochemistry·K SchneiderJ Kottmann
Jun 23, 2015·MicrobiologyOpen·Erin K Heiniger, Caroline S Harwood
Jan 1, 1994·Critical Reviews in Biotechnology·R M AllenV K Shah
Dec 9, 2016·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Daniel SippelOliver Einsle
Mar 14, 2018·Microbiology·Jesse Noar, José M Bruno-Bárcena
Apr 10, 2018·Metallomics : Integrated Biometal Science·Florence MusJohn W Peters
Jun 20, 2018·Applied and Environmental Microbiology·Jace NatzkeJosé M Bruno-Bárcena
Apr 26, 2015·Applied and Environmental Microbiology·Jesse NoarJosé M Bruno-Bárcena
Oct 17, 2018·Molecular Microbiology·Lisa DemtröderBernd Masepohl
May 1, 1988·Molecular Microbiology·L T BennettD R Dean
Jan 18, 2018·Nature Microbiology·Yanning ZhengCaroline S Harwood
Jan 1, 1988·Critical Reviews in Microbiology·R D Joerger, P E Bishop
Mar 22, 2020·Applied and Environmental Microbiology·Jace Natzke, José M Bruno-Bárcena
Jul 15, 2020·Annual Review of Microbiology·Caroline S Harwood
Jul 11, 2017·Nature Chemical Biology·Daniel Sippel, Oliver Einsle
Mar 15, 2018·MBio·Johannes G RebeleinMarkus W Ribbe
Nov 23, 2017·Frontiers in Microbiology·Pedro J Cabello-YevesFrancisco Rodriguez-Valera

Citations

Oct 27, 1975·Biochemical and Biophysical Research Communications·R H SwisherF J Reithel
Mar 30, 1972·Biochimica Et Biophysica Acta·J R BenemannM D Kamen
Jan 1, 1968·Canadian Journal of Microbiology·G W Strandberg, P W Wilson
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G B Ruvkun, F M Ausubel

Related Concepts

Nitrogen Fixation
Tungsten
Sample Fixation
Combined Molybdoflavoprotein Enzyme Deficiency
Blood Ammonia Measurement
Ammonia Inhalants
Mutant
Ammonia
Ammonia Measurement
Azotobacter

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.