Evidence for an iron center in the ammonia monooxygenase from Nitrosomonas europaea

FEBS Letters
J A ZahnAlan A Dispirito

Abstract

Binding of the ligand, nitric oxide, in the presence of reductant was used to identify a ferrous S = 3/2 signal, characteristic of a ferrous nitrosyl complex, and a g= 2.03 copper or iron signal in membranes of the ammonia-oxidizing bacterium, Nitrosomonas europaea. The same ferrous S = 3/2 signal is thought to be a component of the membrane-associated methane monooxygenase (pMMO) of Methylococcus capsulatus Bath, since it is seen in the membrane fraction of cells expressing pMMO and in the purified enzyme, but not in the membrane fraction of cells expressing the soluble MMO [Zahn, J.A. and DiSpirito, A.A. (1996) J. Bacteriol. 178, 1018-1029]. Treatment of resting membranes or cells of N. europaea with nitrapyrin, 2-chloro,6-trichloromethylpyridine, resulted in the increase in magnitude of a g = 6, high-spin ferric iron signal. In the presence of NO and reductant, nitrapyrin prevented the formation of the S = 3/2 nitrosyl-iron complex while increasing the intensity of the g = 6 signal. Nitrapyrin is a specific inhibitor of, and is reduced by, the ammonia monoxygenase (AMO) [Bédard, C. and Knowles, R. (1989) Microbiol. Rev. 53, 68-83]. Taken together the data suggest that iron capable of forming the S = 3/2 complex is a catalyti...Continue Reading

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Citations

Jul 17, 1998·Current Opinion in Chemical Biology·S J Ferguson
May 21, 2009·Biological Chemistry·Stefan GilchIngo Schmidt
Mar 6, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Stefan GilchIngo Schmidt
May 5, 2016·The Journal of Nutritional Biochemistry·Hanna LewandowskaKamil Brzóska
Jun 15, 2011·Systematic and Applied Microbiology·Huiluo CaoJi-Dong Gu
Mar 5, 2014·Chemical Reviews·Edward I SolomonLi Tian
Dec 26, 2003·Critical Reviews in Biochemistry and Molecular Biology·Daniel J Arp, Lisa Y Stein
May 23, 2019·Angewandte Chemie·Sebastian Wendeborn
Oct 6, 1998·Applied and Environmental Microbiology·L Y Stein, D J Arp
Jul 11, 1998·Journal of Bacteriology·A A DiSpiritoA Taylor

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