Evidence for an Na(+)-K(+)-Cl- cotransporter in mammalian type I vestibular hair cells

The American Journal of Physiology
K J RennieM J Correia

Abstract

In amniotes, there are two types of hair cells, designated I and II, that differ in their morphology, innervation pattern, and ionic membrane properties. Type I cells are unique among hair cells in that their basolateral surfaces are almost completely enclosed by an afferent calyceal nerve terminal. Recently, several lines of evidence have ascribed a motile function to type I hair cells. To investigate this, elevated external K+, which had been used previously to induce hair cell shortening, was used to induce shape changes in dissociated mammalian type I vestibular hair cells. Morphologically identified type I cells shortened and widened when the external K+ concentration was raised isotonically from 2 to 125 mM. The shortening did not require external Ca2+ but was abolished when external Cl- was replaced with gluconate or sulfate and when external Na+ was replaced with N-methyl-D-glucamine. Bumetanide (10-100 microM), a specific blocker of the Na(+)-K(+)-Cl- cotransporter, significantly reduced K(+)-induced shortening. Hyposmotic solution resulted in type I cell shape changes similar to those seen with high K+, i.e., shortening and widening. Type I cells became more spherical in hyposmotic solution, presumably as a result of ...Continue Reading

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Citations

Dec 23, 1999·Journal of Hypertension·M TepelW Zidek
Nov 7, 2006·Journal of Cardiovascular Pharmacology·Chao LiuKunshen Liu
Mar 3, 2007·Pharmacological Reviews·Noboru TodaTomio Okamura
Jan 6, 2010·Cardiology Research and Practice·Ayad JazrawiMarcel Zughaib
Apr 9, 2014·Journal of Cardiovascular Pharmacology·Chao LiuUNKNOWN COPE-ADHF Study Group
May 23, 2002·Journal of Hypertension·Leopoldo A Sosa LeónClive N May
Oct 31, 2008·International Heart Journal·Huimin ZhangKunshen Liu
May 5, 2016·Canadian Journal of Physiology and Pharmacology·Chao LiuJian-Xin Zhang
Feb 26, 2011·Experimental Brain Research·Rebecca LimAlan M Brichta
Sep 3, 2008·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Ali RahmanM Nuri Bozdemir

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