Evidence for antigen recognition by nonspecific cytotoxic cells: initiation of 3H-thymidine uptake following stimulation by a protozoan parasite and homologous cognate synthetic peptide

Developmental and Comparative Immunology
D L EvansL Jaso-Friedmann

Abstract

Catfish nonspecific cytotoxic cells bind to and lyse certain protozoan parasites and tumor cells. Target cell binding is facilitated by recognition of (minimally) one antigenic determinant. Binding to this determinant initiates multiple signalling pathways in NCC including protooncogene kinase phosphorylation, regulation of phosphatase activity and increased membrane receptor expression. In the present study, highly purified NCC were activated in vitro with the protozoan parasite Tetrahymena pyriformis, with a multiple antigenic peptide (MAP) composed of the cognate antigenic determinant of this parasite (i.e. natural killer target antigen/NKTag) and NCC were activated with a monoclonal antibody specific for the NCC receptor which binds NKTag. NCC were purified by Percoll density gradients and negative selection by panning (2x) over anti-sIg specific mab 9E1. In 5 day proliferation experiments, treatment of NCC with immobilized Tetrahymena initiated a significant increase in uptake of tritiated thymidine. This appeared to be a primary response in that NCC from in vivo parasite primed catfish did not have secondary-like proliferation responses. Stimulation of NCC with immobilized synthetic peptides composed of the cognate antige...Continue Reading

References

Jul 1, 1989·The Journal of Experimental Medicine·J H PhillipsL L Lanier
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Aug 1, 1994·The Journal of Experimental Medicine·V LitwinL L Lanier
Aug 1, 1997·Molecular Immunology·L Jaso-FriedmannD L Evans

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Citations

Feb 26, 2000·Developmental and Comparative Immunology·L Jaso-FriedmannD L Evans
Aug 28, 2001·Veterinary Parasitology·K BuchmannM Dalgaard
Jan 20, 2006·The Journal of Parasitology·Benjamin J DoddRonald V Dimock
Nov 3, 2005·Veterinary Immunology and Immunopathology·C DautremepuitsM Fournier
Oct 7, 2019·Parasite Immunology·Kurt Buchmann

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