Evidence for Avt6 as a vacuolar exporter of acidic amino acids in Saccharomyces cerevisiae cells

The Journal of General and Applied Microbiology
Thippayarat ChahomchuenYoshimi Kakinuma

Abstract

Here we examined the significance of Avt6, a vacuolar exporter of glutamate and aspartate suggested by the in vitro membrane vesicle experiment, in vacuolar compartmentalization of amino acids in Saccharomyces cerevisiae cells. Fluorescent microscopic observation of GFP-fused Avt6 revealed it to be exclusively localized to the vacuolar membrane, with the amount of Myc-tagged Avt6 significantly increased under nitrogen starvation. Glutamate uptake by cells was enhanced by deletion of the AVT6 gene, indicating indirect involvement of Avt6 in cellular glutamate accumulation. Differences in acidic amino acid content of both total and vacuolar fractions were insignificant between the parent and avt6Delta cells when cultured in nutrient-rich conditions. However, in nitrogen-starved conditions, the amount of glutamate and aspartate in the vacuolar fraction was notably increased in the avt6Delta cells. Avt6 is thus involved in vacuolar amino acid compartmentalization in S. cerevisiae cells, especially under conditions of nitrogen starvation.

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Citations

Mar 16, 2012·Genetics·Per O Ljungdahl, Bertrand Daignan-Fornier
Jun 18, 2015·Bioscience, Biotechnology, and Biochemistry·Siriporn LunpromKoichi Akiyama
Oct 1, 2014·Bioscience, Biotechnology, and Biochemistry·Junichi ToneYoshimi Kakinuma
Mar 10, 2015·Bioscience, Biotechnology, and Biochemistry·Junichi ToneYoshimi Kakinuma
Feb 18, 2011·Molecular Plant·Sakiko Okumoto, Guillaume Pilot
Jul 19, 2014·Bioscience, Biotechnology, and Biochemistry·Takayuki SekitoYoshimi Kakinuma
Sep 18, 2014·Bioscience, Biotechnology, and Biochemistry·Takayuki SekitoYoshimi Kakinuma
Oct 3, 2018·Biological & Pharmaceutical Bulletin·Miyuki Kawano-KawadaTakayuki Sekito
Sep 14, 2012·Bioscience, Biotechnology, and Biochemistry·Masaya IshimotoYoshimi Kakinuma
Mar 22, 2014·The Journal of Toxicological Sciences·Takayuki SekitoYoshimi Kakinuma
Oct 18, 2019·Microbiology and Molecular Biology Reviews : MMBR·Frans BianchiBert Poolman
Feb 25, 2021·Bioscience, Biotechnology, and Biochemistry·Akane SatoTakayuki Sekito
Mar 11, 2021·PLoS Genetics·Raffaele NicastroClaudio De Virgilio
Nov 16, 2020·Biochimica Et Biophysica Acta. Biomembranes·Miyuki Kawano-KawadaTakayuki Sekito

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