Evidence for Bicarbonate Secretion by Ameloblasts in a Novel Cellular Model

Journal of Dental Research
Erzsébet BoriGábor Varga

Abstract

Formation and growth of hydroxyapatite crystals during amelogenesis generate a large number of protons that must be neutralized, presumably by HCO3 (-)ions transported from ameloblasts into the developing enamel matrix. Ameloblasts express a number of transporters and channels known to be involved in HCO3 (-)transport in other epithelia. However, to date, there is no functional evidence for HCO3 (-)transport in these cells. To address questions related to HCO3 (-)export from ameloblasts, we have developed a polarized 2-dimensional culture system for HAT-7 cells, a rat cell line of ameloblast origin. HAT-7 cells were seeded onto Transwell permeable filters. Transepithelial resistance was measured as a function of time, and the expression of transporters and tight junction proteins was investigated by conventional and quantitative reverse transcription polymerase chain reaction. Intracellular pH regulation and HCO3 (-)transport were assessed by microfluorometry. HAT-7 cells formed epithelial layers with measureable transepithelial resistance on Transwell permeable supports and expressed claudin-1, claudin-4, and claudin-8-key proteins for tight junction formation. Transport proteins previously described in maturation ameloblasts ...Continue Reading

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Citations

Jul 17, 2016·European Journal of Oral Sciences·Antonius L J J BronckersPamela K DenBesten
Feb 22, 2017·Journal of Dental Research·A L J J Bronckers
May 5, 2017·Physiological Reviews·Rodrigo S LacruzMichael L Paine
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Jan 6, 2019·International Journal of Oral Science·Mirali Pandya, Thomas G H Diekwisch
Sep 2, 2020·The Journal of Biological Chemistry·Yuta ChibaSatoshi Fukumoto
May 29, 2018·Archives of Oral Biology·Hee-Eun Kim, Jeong Hee Hong
May 1, 2021·International Journal of Molecular Sciences·Kristóf KádárÁkos Zsembery
Jun 3, 2021·Materials·Mihaela OlaruGabriela Calin

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