Evidence for Brassinosteroid-Mediated PAT During Germination of Spathoglottis plicata (Orchidaceae)
Abstract
Polar auxin transport (PAT) is facilitated by polar localization of PIN-FORMED (PIN) efflux carriers, which direct auxin flow and regulate developmental events. Brassinosteroids (BRs) and auxin work synergistically to promote growth, and in root geotropisms this cross-talk involves BR-directed polarization of PIN through the mobilization of F-actin. However, the role of BR in PAT during shoot growth, hair formation, and embryogenesis has not been well studied. Orchid seed are mature at a point in development that is analogous to the globular-stage of embryogenesis in typical angiosperms. Thus, this system provided a unique opportunity to study the effects of BR on PAT during embryogenesis-like events, including meristem/first leaf formation and protocorm/stem development, which is followed by protocorm hair formation. In this work, the degree to which BRs rescued embryo-like protocorms from the impact of PAT-disrupting agents, such as PAT inhibitors or high auxin levels, was determined based on growth responses. This study first established that auxin and BRs work together synergistically to promote seedling elongation in Spathoglottis. Repressed seedling growth caused by the PAT-disrupting agents was alleviated with eBL, sugge...Continue Reading
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