PMID: 375008Jan 31, 1979Paper

Evidence for co-transcription of the RNA polymerase genes rpoBC with a ribosomal protein gene of escherichia coli

Molecular & General Genetics : MGG
A J NewmanR S Hayward

Abstract

The adjacent genes rpoB and rpoC code for the beta and beta' subunits of RNA polymerase in Escherichia coli, and are cotranscribed in the order given. The nearest known genes to rpoB are rplL and rplA,J,K, which code for ribosomal proteins, and which are transcribed in the same direction as the polymerase genes. It has been suggested that rpoBC may be distal elements of a larger operon including these ribosomal genes. To test this possibility we have cloned a segment of DNA, derived by endoR. HindIII digestion from the rpoBC-transducing bacteriophage lambdarifd18, in the replacement vector NMlambda761. The structure of the lambdarpoBC bacteriophages so produced is such that the inserted DNA can be transcribed from lambda promoters, allowing us to confirm that it carries intact rplL, rpoB, and rpoC genes. We have studied these bacteriophages as lysogens in rec+ and rec bacteria, and by infection of UV-irradiated bacterial strains in which lambda promoters are either repressed or active. The results indicate that the cloned DNA contains at most a very weak promoter for the above genes, in contrast to that present in the larger segment of bacterial DNA carried by lambdarifd18. We have in the same way cloned the adjacent bacterial ...Continue Reading

References

Jan 7, 1977·Molecular & General Genetics : MGG·N E MurrayK Murray
Jan 1, 1977·Nucleic Acids Research·J R CameronR W Davis
Oct 20, 1977·Molecular & General Genetics : MGG·S M Schweitzer, H Matzura
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·P P Dennis
Mar 20, 1978·Molecular & General Genetics : MGG·R S Hayward, S K Fyfe
Dec 1, 1977·Molecular Biology Reports·I Boros, B Sain
May 7, 1976·Molecular & General Genetics : MGG·I P Tittawella
Jul 23, 1976·Molecular & General Genetics : MGG·K BorckN E Murray
Oct 3, 1975·Molecular & General Genetics : MGG·S J Hardy
Jan 1, 1968·Cold Spring Harbor Symposia on Quantitative Biology·M E Gottesman, M B Yarmolinsky

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Citations

Feb 1, 1980·Molecular & General Genetics : MGG·A Newman, R S Hayward
Mar 1, 1986·Molecular & General Genetics : MGG·D W Meek, R S Hayward
Jan 1, 1981·Molecular & General Genetics : MGG·J C MaR S Hayward
Jan 1, 1980·Molecular & General Genetics : MGG·S J Dowsett, B H Sells
Apr 13, 1981·Molecular and Cellular Biochemistry·N Brot, H Weissbach
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G BarryC Squires
Dec 28, 1979·Biochemical and Biophysical Research Communications·G GoldbergN Brot
Apr 20, 1987·Journal of Molecular Biology·W L Downing, P P Dennis
Jun 1, 1981·Cell·F S Young, A V Furano
Dec 1, 1982·Journal of Bacteriology·I HuiP Dennis
Nov 1, 1982·Journal of Bacteriology·S P Ridley, M P Oeschger
Nov 1, 1979·Journal of Bacteriology·G An, J D Friesen
Mar 1, 1980·Microbiological Reviews·B J Bachmann, K B Low

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