Evidence for common mechanisms in the transcriptional control of type II nitric oxide synthase in isolated hepatocytes. Requirement of NF-kappaB activation after stimulation with bacterial cell wall products and phorbol esters.

The Journal of Biological Chemistry
M J Díaz-GuerraL Boscá

Abstract

Incubation of primary cultures of rat hepatocytes with lipopolysaccharide (LPS), S-[2,3-bis(palmitoyloxy)-(2-R, S)-propyl]-N-palmitoyl-(R)-Cys-Ser-Lys4 (TPP), a synthetic lipopeptide present in bacterial cell wall lipoproteins, or with phorbol 12,13-dibutyrate (PDBu) induced an increase in nitric oxide synthesis through the expression of type II nitric oxide synthase (iNOS). Transfection of hepatocytes with a HindII fragment corresponding to the promoter region of the murine iNOS gene (from nucleotide -1588 to +165) resulted in the expression of the reporter gene when cells were stimulated with these factors. The transcription factors activated by these stimuli involved an increase in the nuclear content of proteins that bind to kappaB, AP-1, GAS, and SIE sequences. Inhibition of NF-kappaB activation with pyrrolidine dithiocarbamate eliminated the expression of iNOS in hepatocytes stimulated with LPS, TPP, or PDBu. In addition to this, transfection of hepatocytes with promoter mutants in which a sequential 2-base pair change within the kappaB sites was introduced (position -971 to -961 and -85 to -75, respectively), resulted in approximately 17 and 35%, respectively, of the activity of the naive promoter. Simultaneous mutation ...Continue Reading

References

May 1, 1992·The Journal of Experimental Medicine·R SchreckP A Baeuerle
Jun 1, 1992·Research in Immunology·W G Bessler, G Jung
Sep 1, 1982·Molecular and Cellular Biology·C M GormanB H Howard
Sep 1, 1994·The Journal of Experimental Medicine·E MartinQ W Xie
Sep 23, 1994·Cell·C Nathan, Q W Xie
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D A GellerT R Billiar
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·C J LowensteinW J Murphy
Mar 1, 1995·Trends in Biochemical Sciences·E Cano, L C Mahadevan
Jun 9, 1995·The Journal of Biological Chemistry·H B PengJ K Liao
Feb 24, 1995·Cell·D Thanos, T Maniatis
Jan 1, 1994·Annual Review of Immunology·P A Baeuerle, T Henkel
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·P GhoshH A Young
May 29, 2013·Molecular Systems Biology·Mathieu CourcellesPierre Thibault

❮ Previous
Next ❯

Citations

Jan 27, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T A VosH Moshage
Apr 15, 2005·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·E R WooK W Kang
Mar 7, 2003·Nitric Oxide : Biology and Chemistry·Emilio SiendonesJordi Muntané
Oct 12, 1999·British Journal of Pharmacology·B de las HerasA Villar
Feb 5, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Nuria A CallejasPaloma Martín-Sanz
Oct 24, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Kerstin KoerberGisa Tiegs
Jan 7, 2003·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Paloma Martín-SanzLisardo Boscá
Apr 1, 2003·Animal Health Research Reviews·Robert P Hunter
Jul 19, 2000·The EMBO Journal·L B MaggiJ A Corbett
May 3, 2006·Biological & Pharmaceutical Bulletin·Eun-Rhan WooKeon Wook Kang
May 13, 2014·Biological & Pharmaceutical Bulletin·Xing LinQuanfang Huang
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·R M RaiA M Diehl
Jul 29, 2004·American Journal of Respiratory and Critical Care Medicine·UNKNOWN ad hoc Statement Committee, American Thoracic Society
Apr 10, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Kyung Hee JungSung-Vin Yim
Jun 11, 2004·Liver International : Official Journal of the International Association for the Study of the Liver·Dalia FouadJordi Muntané
Feb 28, 2004·Insect Molecular Biology·S J HuangWen-Long Cho
Nov 18, 2005·Journal of Ethnopharmacology·Jin Won YangKeon Wook Kang
Jan 27, 2010·Reproductive Biology and Endocrinology : RB&E·Sonali VoraWilliam E Ackerman
Jun 5, 2003·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·José L M MadrigalJuan C Leza
Feb 7, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·X W QuW Hsueh
Nov 27, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Emilio SiendonesJordi Muntané

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.