PMID: 6113271Jun 1, 1981Paper

Evidence for compartmentation of synaptosomal phosphate-activated glutaminase

Journal of Neurochemistry
E Kvamme, B E Olsen

Abstract

Phosphate-activated glutaminase (EC 3.5.1.2) in synaptosomal preparations is inhibited 40-60% by the sulphydryl group reagent N-ethylmaleimide (NEM), forming the basis for distinction between NEM-sensitive and NEM-insensitive glutaminases. The NEM effect cannot be explained by differential effects on distinct glutaminases because other glutaminases have not been detected, and the synaptosomal glutaminase activity can be fully accounted for by the activity of phosphate-activated glutaminase. By fractionation of mitochondria isolated from synaptosomal preparations, which are preincubated with and without NEM, both NEM-sensitive and NEM-insensitive glutaminases are found to be localized to the inner mitochondrial membrane. Variations in pH (7.0-7.6) and the phosphate concentration (5-10 mM) affect chiefly NEM-sensitive glutaminase, demonstrating that this glutaminase may be subject to regulation by compounds in the cytosol having restricted permeability to the inner mitochondrial membrane. Since p-hydroxymercuribenzoate, which is known to be impermeable to the inner mitochondrial membrane, inhibits glutaminase similarly to NEM, phosphate-activated glutaminase is assumed to be compartmentalized within the inner mitochondrial membra...Continue Reading

References

Nov 1, 1979·Journal of Neurochemistry·G A Nimmo, K F Tipton
Jan 1, 1979·Advances in Experimental Medicine and Biology·E Kvamme
Jun 1, 1978·Journal of Neurochemistry·H F BradfordA J Thomas
Feb 15, 1976·The Biochemical Journal·J C Lai, J B Clark
Jun 25, 1976·Brain Research·H F Bradford, H K Ward
Jul 1, 1966·Archives of Biochemistry and Biophysics·E BachmannD E Green
Nov 1, 1971·Journal of Neurochemistry·G Svenneby
Jan 1, 1968·Advances in Enzyme Regulation·N KatunumaY Matsuda
Sep 8, 1965·Biochemical and Biophysical Research Communications·E KvammeG Svenneby
Nov 1, 1978·Neuroscience Letters·J C ReubiM Cuénod

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Citations

Dec 18, 2001·Journal of Neuroscience Research·E KvammeB Roberg
Dec 2, 2004·Journal of Neuroscience Research·K V Rama RaoM D Norenberg
Feb 1, 1983·Journal of Neuroscience Methods·J Butterworth, C M Yates
Jan 1, 1986·Comparative Biochemistry and Physiology. A, Comparative Physiology·A J PotterD G Drescher
Jan 1, 1983·Progress in Neurobiology·E Kvamme
Jan 1, 1990·Progress in Neurobiology·M Erecińska, I A Silver
Aug 1, 1992·Progress in Neurobiology·J C Szerb, R F Butterworth
May 17, 2000·Neurochemistry International·E KvammeI A Torgner
Dec 1, 1986·Neuropharmacology·A D Sherman, J Mott
Jun 1, 1988·Neurochemical Research·A D ShermanJ Mott
Jan 1, 1983·Neurochemical Research·E KvammeI A Torgner
Feb 1, 1993·Neurochemical Research·R Dawson, D R Wallace
Aug 26, 2009·The International Journal of Biochemistry & Cell Biology·José M MatésJavier Márquez
Aug 1, 1989·Journal of Neurochemistry·M YudkoffM Erecińska
Aug 1, 1983·Journal of Neurochemistry·J ButterworthJ Simpson
Jan 24, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·S Sahai
Mar 1, 1983·Journal of Neurochemistry·H K WardH F Bradford
Mar 30, 2004·Glia·Arumugam R JayakumarMichael D Norenberg
Sep 1, 1983·Journal of Neurochemistry·C M ThankiH F Bradford
May 20, 2004·Journal of Inorganic Biochemistry·Lidia Struzyńska, Grzegorz Sulkowski
Nov 15, 1985·Archives of Biochemistry and Biophysics·R A ShapiroN P Curthoys

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