Evidence for compromised metabolic function and limited glucose uptake in spermatozoa from the teratospermic domestic cat (Felis catus) and cheetah (Acinonyx jubatus).

Biology of Reproduction
Kimberly A TerrellAdrienne Crosier

Abstract

Cheetahs and certain other felids consistently ejaculate high proportions (≥ 60%) of malformed spermatozoa, a condition known as teratospermia, which is prevalent in humans. Even seemingly normal spermatozoa from domestic cat teratospermic ejaculates have reduced fertilizing capacity. To understand the role of sperm metabolism in this phenomenon, we conducted a comparative study in the normospermic domestic cat versus the teratospermic cat and cheetah with the general hypothesis that sperm metabolic function is impaired in males producing predominantly pleiomorphic spermatozoa. Washed ejaculates were incubated in chemically defined medium containing glucose and pyruvate. Uptake of glucose and pyruvate and production of lactate were assessed using enzyme-linked fluorescence assays. Spermatozoa from domestic cats and cheetahs exhibited similar metabolic profiles, with minimal glucose metabolism and approximately equimolar rates of pyruvate uptake and lactate production. Compared to normospermic counterparts, pyruvate and lactate metabolism were reduced in teratospermic cat and cheetah ejaculates, even when controlling for sperm motility. Rates of pyruvate and lactate (but not glucose) metabolism were correlated positively with sp...Continue Reading

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Citations

Nov 4, 2016·Reproduction in Domestic Animals = Zuchthygiene·K Chatdarong
Dec 3, 2016·General and Comparative Endocrinology·Diana C KoesterAdrienne E Crosier
Feb 5, 2019·Biology of Reproduction·Halli S WeinerCarol L Keefer
Sep 22, 2015·Reproduction, Fertility, and Development·Diana C KoesterAdrienne E Crosier
Jan 22, 2021·Animal Reproduction Science·Audra A HuffmeyerRobert K Wayne
Jan 4, 2017·Theriogenology·Santiago Jimenez GonzalezClaudia Jimenez Escobar

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Methods Mentioned

BETA
SMI
enzyme-linked fluorescence

Software Mentioned

SoftMax Pro
Statistical Analysis Software ( SAS )
SAS

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