Evidence for conserved function of γ-glutamyltranspeptidase in Helicobacter genus.

PloS One
Mirko RossiMarja-Liisa Hänninen

Abstract

The confounding consequences of Helicobacter bilis infection in experimental mice populations are well recognized, but the role of this bacterium in human diseases is less known. Limited data are available on virulence determinants of this species. In Helicobacter pylori, γ-glutamyltranspeptidase (γGT) contributes to the colonization of the gastric mucosa and to the pathogenesis of peptic ulcer. The role of γGT in H. bilis infections remains unknown. The annotated genome sequence of H. bilis revealed two putative ggt genes and our aim was to characterize these H. bilis γGT paralogues. We performed a phylogenetic analysis to understand the evolution of Helicobacter γGTs and to predict functional activities of these two genes. In addition, both copies of H. bilis γGTs were expressed as recombinant proteins and their biochemical characteristics were analysed. Functional complementation of Esherichia coli deficient in γGT activity and deletion of γGT in H. bilis were performed. Finally, the inhibitory effect of T-cell and gastric cell proliferation by H. bilis γGT was assessed. Our results indicated that one gene is responsible for γGT activity, while the other showed no γGT activity due to lack of autoprocessing. Although both H. ...Continue Reading

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Citations

Feb 15, 2014·BMC Genomics·Caroline P A Skarp-de HaanMirko Rossi
Nov 16, 2013·Antonie van Leeuwenhoek·Namrata Kushwaha, Sheela Srivastava
Sep 14, 2012·Helicobacter·Robin M Delahay, Massimo Rugge
Sep 14, 2012·Helicobacter·Mirko Rossi, Marja-Liisa Hänninen
Jan 18, 2018·Infection and Immunity·Robin L Cassady-CainMark P Stevens
May 18, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Romy KäbischRaquel Mejías-Luque

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Methods Mentioned

BETA
PCR
Circular Dichroism
ELISA
FACS
electrophoresis
Fluorescence-activated cell sorting

Software Mentioned

PSORTb
PHYML
CELLO
iCODEHOP
FindModel
SignalP
MEGA4
SWAKK
FloJo
GraphPad

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