PMID: 6271215Sep 15, 1981Paper

Evidence for convertible forms of soluble uterine cyclic nucleotide phosphodiesterase

Biochimica Et Biophysica Acta
S J StradaG M Stancel


The cyclic nucleotide phosphodiesterase (3':5'-cyclic nucleotide 5'-nucleotidohydrolase, EC systems of many tissues show multiple physical and kinetic forms. In contrast, the soluble rat uterine phosphodiesterase exists as a single enzyme form with non-linear Lineweaver-Burk kinetics for cyclic AMP (app. Km of approx. 3 and 20 microM) and linear kinetics for cyclic GMP (app. Km of approx. 3 microM) since the two hydrolytic activities are not separated by a variety of techniques. In uterine cytosolic fractions, cyclic AMP is a non-competitive inhibitor of cyclic GMP hydrolysis (Ki approx. 32 microM). Also, cyclic GMP is a non-competitive inhibitor of cyclic AMP hydrolysis (Ki approx 16 microM) at low cyclic GMP/cyclic AMP substrate ratios. However, cyclic GMP acts as a competitive inhibitor of cyclic AMP phosphodiesterase (Ki approx 34 microM) at high cyclic GMP/cyclic AMP substrate ratios. When a single hydrolytic form of uterine phosphodiesterase, separated initially by DEAE anion-exchange chromatography, is treated with trypsin (0.5 microgram/ml for 2 min) and rechromatographed on DEAE-Sephacel, two major forms of phosphodiesterase are revealed. One form elutes at 0.3 M NaOAc- and displays anomalous kinetics for cyc...Continue Reading


Mar 1, 1975·Metabolism: Clinical and Experimental·W L Terasaki, M M Appleman
Oct 1, 1975·Molecular and Cellular Endocrinology·G M StancelS J Strada
Aug 1, 1976·Archives of Biochemistry and Biophysics·R G Van InwegenW J Thompson
Jul 25, 1978·Biochemistry·E A GardnerG M Stancel
Dec 8, 1978·Biochimica Et Biophysica Acta·P M EpsteinS J Strada
Jan 19, 1971·Biochemistry·W J Thompson, M M Appleman
Jul 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·T R RussellM M Appleman
Dec 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·R C BhallaS G Korenman
Mar 8, 1973·Life Sciences. Pt. 2: Biochemistry, General and Molecular Biology·G Angles d'Auriac, P Meyer
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·F A KuehlN D Goldberg
Feb 1, 1973·Analytical Biochemistry·G R Schacterle, R L Pollack
Sep 1, 1980·The Biochemical Journal·T ItanoJ T Penniston
Aug 1, 1953·The Biochemical Journal·M DIXON

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