Evidence for cyclooxygenase-1 association with caveolin-1 and -2 in cultured human embryonic kidney (HEK 293) cells

IUBMB Life
Seok Ho ChaJin-Oh Kwak

Abstract

The purpose of this study was to confirm protein-protein interaction between cyclooxygenase-1 (COX-1) and caveolins. The interaction of cyclooxygenase-1 and caveolins in the cultured human embryonic kidney (HEK 293) cells was investigated using immuno-precipitation and Western blot analysis. In HEK 293 cells, high levels of caveolin-2 and low level of caveolin-1 at mRNA and protein level were observed without any detectable expression of caveolin-3. Caveolae rich membranous fractions from the HEK 293 cells contained both COX-1 and caveolin-1 or caveolin-2 in same fractions. The experiments of immuno-precipitation showed complex formation between the COX-1 and caveolin-1 or caveolin-2 in the HEK 293 cells. Confocal microscopic results also support co-localization of COX-1 and caveolin-1 or caveolin-2 at the plasma membrane. Co-localization of caveolins with cylooxygenase-1 in caveolae suggested that caveolin would play an important role in regulating the function of COX-1.

Citations

Sep 23, 2008·Metabolism: Clinical and Experimental·Lei WangBernhard Hennig
Jul 29, 2008·The Journal of Nutritional Biochemistry·Yuanyuan ZhengBernhard Hennig
Feb 16, 2006·Journal of Korean Medical Science·Jin-Oh KwakSeok Ho Cha
May 19, 2007·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·David W L Ma
Mar 30, 2020·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Mutay AslanÇağatay Yılmaz

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