Evidence for de novo synthesis of phosphatidylinositol coupled with histamine release in activated rat mast cells

Biochemical and Biophysical Research Communications
Y IshizukaY Nozawa

Abstract

Phospholipid metabolisms in rat mast cells activated by ionophore A23187 and compound 48/80 were examined with reference to 'phosphatidylinositol (PI) cycle'. The addition of A23187 to [3H]glycerol-prelabeled mast cells induced a marked accumulation of the radioactivity in 1,2-diacylglycerol(DG) and phosphatidic acid(PA) within 10 to 30 sec. A great enhancement of [3H]glycerol incorporation into PA and PI was also detected during histamine release. On the other hand, 48/80 was far less effective than A23187 both in producing 1,2- DG and PA and in accerelating [3H]glycerol incorporation into PA and PI, despite the comparable ability of histamine release. The activity of Ca2+ uptake into mast cells, as measured by pulse-labeling with 45Ca2+, was increased when exposed to both of two agents. These data provide circumstantial evidence that phospholipid metabolisms, mainly de novo PI synthesis, may be a part of the triggering events for Ca2+ mobilization and secretory process. The PI metabolism induced by two different stimulants appears to behave in a different manner.

References

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Citations

Jul 12, 1991·European Journal of Pharmacology·T KomabayashiM Tsuboi
Mar 12, 1992·European Journal of Pharmacology·T KomabayashiM Tsuboi
Jan 1, 1987·Pharmacology & Toxicology·T Johansen
Feb 13, 1987·Biochemical and Biophysical Research Communications·S NagaoY Nozawa
Jan 16, 1985·Biochemical and Biophysical Research Communications·M W Musch, M I Siegel
Jun 30, 1986·Biochemical and Biophysical Research Communications·Y OkanoY Nozawa
Mar 29, 1985·Biochemical and Biophysical Research Communications·Y OkanoY Nozawa

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