Evidence for differences in the metabolism of saxitoxin and C1+2 toxins in the freshwater cyanobacterium Cylindrospermopsis raciborskii T3

Biochimica Et Biophysica Acta
Francesco PomatiBrett Anthony Neilan

Abstract

The activity of paralytic shellfish poisoning (PSP) toxins biosynthetic enzymes was assayed in the cyanobacterium Cylindrospermopsis raciborskii T3 after inhibiting protein synthesis with chloramphenicol (CAM). The production of C1+2 and saxitoxin (STX) was sensitive to CAM with STX levels decreasing by 70% after 24-h exposure to the antibiotic. PSP toxin production was strongly promoted by arginine supplementation, with a maximum 476% increase in intracellular STX concentrations after 24-h exposure to 10 mM of the amino acid. However, arginine had no stimulating effect on PSP toxin levels if supplemented in combination with CAM at 10 microg l(-1). Addition of agmatine and proline to C. raciborskii T3 cultures in the presence of 10 microg l(-1) CAM increased C1+2 toxins levels, while having a negative or no effect on STX accumulation. In vitro, PSP toxin levels increased naturally in cyanobacterial extracts, with CAM and arginine having no influence on either C1+2 or STX synthesis. The evidence presented in this study suggests a possible difference between the metabolism of STX and the C1+2 toxins and indicated a high turnover rate of STX biosynthetic enzymes in C. raciborskii T3.

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Dec 9, 2003·Applied and Environmental Microbiology·Francesco PomatiBrett A Neilan

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Citations

Oct 12, 2010·Applied and Environmental Microbiology·Jamal Al-TebrinehBrett A Neilan
Aug 18, 2010·Marine Drugs·Maria WieseBrett A Neilan
Aug 8, 2009·Toxicon : Official Journal of the International Society on Toxinology·Rómulo AráozNicole Tandeau de Marsac
Apr 21, 2009·Toxicon : Official Journal of the International Society on Toxinology·Shauna A MurrayBrett A Neilan
Apr 29, 2006·Environmental Toxicology·Yongmei LiuLirong Song
Jun 21, 2015·Toxicon : Official Journal of the International Society on Toxinology·Francine HarlandSally Gaw
Jan 13, 2011·Toxicon : Official Journal of the International Society on Toxinology·Francine M J SmithSally Gaw
Aug 16, 2006·FEMS Microbiology Ecology·Céline BergerCécile Bernard
Dec 2, 2019·Toxicon : Official Journal of the International Society on Toxinology·Hong-Nan HuangJie Lin
Jan 25, 2018·Journal of Natural Products·Janusz J PetkowskiSara Seager

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