Evidence for different, host-dependent functioning of Rx against both wild-type and recombinant Pepino mosaic virus

Molecular Plant Pathology
Celia R A Duff-FarrierGary D Foster

Abstract

The potato Rx gene provides resistance against Pepino mosaic virus (PepMV) in tomato; however, recent work has suggested that the resistance conferred may not be durable. Resistance breaking can probably be attributed to multiple mutations observed to accumulate in the capsid protein (CP) region of resistance-breaking isolates, but this has not been confirmed through directed manipulation of an infectious PepMV clone. The present work describes the introduction of two specific mutations, A-T78 and A-T114, into the coat protein minimal elicitor region of an Rx-controlled PepMV isolate of the EU genotype. Enzyme-linked immunosorbent assay (ELISA) and phenotypic evaluation were conducted in three Rx-expressing and wild-type solanaceous hosts: Nicotiana benthamiana, Nicotiana tabacum and Solanum lycopersicum. Mutation A-T78 alone was sufficient to confer Rx-breaking activity in N. benthamiana and S. lycopersicum, whereas mutation A-T114 was found to be associated, in most cases, with a secondary A-D100 mutation to break Rx-mediated resistance in S. lycopersicum. These results suggest that the need for a second, fitness-restoring mutation may be dependent on the PepMV mutant under consideration. Both mutations conferred Rx breaking ...Continue Reading

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Aug 19, 2014·Molecular Plant Pathology·Celia R A Duff-FarrierGary D Foster

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Citations

Dec 23, 2017·Plant Biotechnology Journal·Helen C BrewerGary D Foster
Sep 23, 2018·International Journal of Molecular Sciences·Jean-Luc GalloisSylvie German-Retana
Sep 16, 2018·Viruses·Maathavi KannanHamidun Bunawan

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