PMID: 8965643Mar 1, 1996Paper

Evidence for different mechanisms of induction of HSP70i: a comparison of cultured rat cortical neurons with astrocytes

Brain Research. Molecular Brain Research
R N Nishimura, B E Dwyer

Abstract

This study is a follow-up of previous work which demonstrated that cultured cortical neurons did not synthesize HSP70i immediately after heat stress when compared with cultured cortical astrocytes. We have extended the period of observation for HSP70i induction of cultured cortical neurons and astrocytes up to 24 h after heat stress. Cultured rat cortical neurons derived from 16-day-old fetal rats respond differently to heat stress than cultured rat astrocytes derived from newborn rats. They showed a delayed HSP70i induction in the majority of cultured neurons and the response was heterogeneous and was absent in most smaller neurons. The delayed neuronal induction was accompanied by a prolonged activation of heat-shock transcription factor 1 (HSF-1) and prolonged transcription of HSP70i mRNA. In comparison astrocytes showed a marked early induction of HSP70i mRNA and protein. In addition the induction of HSP70i in astrocytes was followed by translocation of the protein into the nucleus, a finding which we failed to demonstrate in neurons. Immunostaining for HSP70i was more uniform in astrocytes than neurons. Many neurons did not stain for up to 24 h after heat shock in this study. Immunocytochemical staining of HSF-1 and 2 show...Continue Reading

References

Jul 1, 1992·Brain Pathology·B E Dwyer, R N Nishimura
Jan 1, 1991·Brain Research. Molecular Brain Research·R N NishimuraJ de Vellis
Jul 1, 1989·Brain Research. Molecular Brain Research·M F GonzalezF R Sharp
Jun 1, 1994·Cell Biology International·A VenetianerA Hever-Szabo
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Jan 1, 1993·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·H TannoL J Noble

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Citations

Sep 3, 2002·Journal of Neuroscience Research·Araceli Espinosa-JeffreyJean de Vellis
Mar 19, 2008·Cell Stress & Chaperones·Jay OzaAlice Y-C Liu
Oct 3, 1999·European Journal of Biochemistry·Y KawazoeA Nakai
Apr 25, 2006·Brain Research·James E HansenRobert N Nishimura
Jul 16, 2011·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Lesley ChengPhillip Nagley
Jan 18, 2014·Journal of Cellular Physiology·Diana J LiuAlice Y-C Liu
Sep 20, 2017·Molecular Neurodegeneration·Rebecca San GilHeath Ecroyd
May 23, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·R H WeisbartR Nishimura
Mar 5, 2008·Brain Research·Jingxian YangAlice Y-C Liu

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