Evidence for differential storage of calcitonin gene-related peptide, substance P and serotonin in synaptosomal vesicles of rat spinal cord.

Brain Research
G FriedT Hökfelt

Abstract

Homogenates of rat ventral and dorsal spinal cords were subjected to differential and gradient centrifugation on linear sucrose gradients following lysis of a synaptosomal fraction. The distribution of calcitonin gene-related peptide (CGRP) and substance P (SP) in dorsal spinal cord as well as the distribution of serotonin (5-HT) and SP in ventral spinal cord was determined, using radioimmunoassay (RIA) for CGRP and SP and high-performance liquid chromatography for 5-HT. In dorsal spinal cord. CGRP and SP had an almost identical distribution, with one peak in an intermediate density fraction which according to electron microscopy contained i.a. large dense-cored vesicles, and a second peak in a heavy fraction enriched in synaptosome-like structures. In ventral spinal cord, 5-HT and SP had different distribution patterns; 5-HT peaked in a light fraction containing many small synaptic vesicles, whereas SP peaked in an intermediate density fraction similar to the one in dorsal spinal cord. Both 5-HT and SP had second peaks in high density fractions containing synaptosome-like structures. The study demonstrates that the peptides CGRP and SP, partly coexisting in dorsal spinal cord, both seem to be stored in large vesicles inside sy...Continue Reading

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Citations

Mar 8, 2000·Progress in Neurobiology·J F HerreroJ A López-García
Sep 30, 1998·The European Journal of Neuroscience·G JacobssonB Meister
Mar 22, 1996·Annals of the New York Academy of Sciences·C J Helke, L Yang
Jan 31, 2009·The Journal of Comparative Neurology·Maureen S RiedlLaura S Stone
Jun 2, 2007·Journal of Neurochemistry·Cory J Goracke-PostleCarolyn A Fairbanks
Jan 18, 2007·Journal of Neurochemistry·Cory J Goracke-PostleCarolyn A Fairbanks
Aug 1, 1997·Acta Odontologica Scandinavica·I Fristad
Dec 20, 2005·Neuroreport·Cory J Goracke-PostleCarolyn A Fairbanks
Feb 1, 1993·The Journal of Comparative Neurology·A M Williamson, H J Ralston
Aug 28, 1992·Brain Research·J FranckT Hökfelt

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