Oct 16, 1989

Evidence for differential storage of calcitonin gene-related peptide, substance P and serotonin in synaptosomal vesicles of rat spinal cord

Brain Research
G FriedT Hökfelt

Abstract

Homogenates of rat ventral and dorsal spinal cords were subjected to differential and gradient centrifugation on linear sucrose gradients following lysis of a synaptosomal fraction. The distribution of calcitonin gene-related peptide (CGRP) and substance P (SP) in dorsal spinal cord as well as the distribution of serotonin (5-HT) and SP in ventral spinal cord was determined, using radioimmunoassay (RIA) for CGRP and SP and high-performance liquid chromatography for 5-HT. In dorsal spinal cord. CGRP and SP had an almost identical distribution, with one peak in an intermediate density fraction which according to electron microscopy contained i.a. large dense-cored vesicles, and a second peak in a heavy fraction enriched in synaptosome-like structures. In ventral spinal cord, 5-HT and SP had different distribution patterns; 5-HT peaked in a light fraction containing many small synaptic vesicles, whereas SP peaked in an intermediate density fraction similar to the one in dorsal spinal cord. Both 5-HT and SP had second peaks in high density fractions containing synaptosome-like structures. The study demonstrates that the peptides CGRP and SP, partly coexisting in dorsal spinal cord, both seem to be stored in large vesicles inside sy...Continue Reading

Mentioned in this Paper

Pathologic Cytolysis
CALCA gene
Calcitonin Gene-Related Peptide
Amines
CALCA wt Allele
Rat Spinal Cord
Serotonin Measurement
Malignant Neoplasm of Spinal Cord
August Rats
Serotonin

About this Paper

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