PMID: 11908853Mar 23, 2002Paper

Evidence for direct interaction between actin and the cystic fibrosis transmembrane conductance regulator

European Biophysics Journal : EBJ
Bernard ChasanHoracio F Cantiello

Abstract

Previous studies have demonstrated that actin filament organization controls the cystic fibrosis transmembrane conductance regulator (CFTR) ion channel function. The precise molecular nature of the interaction between actin and CFTR, however, remains largely unknown. In this report, interactions between actin and purified human epithelial CFTR were directly assessed by reconstitution of the channel protein in a lipid bilayer system and by atomic force microscopy (AFM). CFTR-containing liposomes in solution were deposited on freshly cleaved mica and imaging was performed in tapping-mode AFM. CFTR function was also determined in identical preparations. Images of single CFTR molecules were obtained, and addition of monomeric actin below its critical concentration showed the formation of actin filaments associated with CFTR. The data indicate a direct interaction between actin and CFTR exists, which may explain the regulatory role of the cytoskeleton in ion channel function. This was confirmed by functional studies of CFTR single-channel currents, which were regulated by addition of various conformations of actin. The present study indicates that CFTR may directly bind actin and that this interaction helps affect the functional pro...Continue Reading

Citations

Dec 7, 2007·Pflügers Archiv : European journal of physiology·Hermann Schillers
Mar 26, 2004·Biophysical Journal·J A TuszyńskiH F Cantiello
Apr 4, 2003·Life Sciences·José Luis Alonso, Wolfgang H Goldmann
Jun 22, 2011·Proceedings of the National Academy of Sciences of the United States of America·Alison M ForsythHoward A Stone
Nov 20, 2012·The Journal of Biological Chemistry·Qian WangXing-Zhen Chen
Nov 18, 2004·Journal of the National Cancer Institute·Francesca LucianiStefano Fais
Jul 8, 2008·BMC Developmental Biology·J Craig CohenKen-Ichi Takemaru
Feb 19, 2016·Pflügers Archiv : European journal of physiology·Alejandra G PalmaGabriela I Marino
Apr 7, 2010·European Journal of Pharmacology·Masahiro MoriseYoshinori Hasegawa
Jan 24, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J Craig CohenAnil Mehta
Jul 22, 2004·Experimental Cell Research·Kathryn L HoweDerek M McKay
Feb 26, 2015·Cell Biology International·Pascal TrouvéClaude Férec
Nov 7, 2006·Biochimica Et Biophysica Acta·Radhika GaneshanKevin L Kirk
Feb 20, 2004·American Journal of Physiology. Cell Physiology·Xiao-Gang LaiTak-Ming Wong
Sep 22, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Jiandi WanHoward A Stone
Feb 1, 2008·Proteomics. Clinical Applications·Franck BrouillardMario Ollero
Dec 17, 2005·The Journal of Biological Chemistry·Christopher MazzochiDale J Benos
Aug 24, 2006·American Journal of Physiology. Renal Physiology·C MazzochiP R Smith
Feb 11, 2020·Biomechanics and Modeling in Mechanobiology·Robert J Asaro, Qiang Zhu
Apr 9, 2011·American Journal of Respiratory Cell and Molecular Biology·Yoshitaka HibinoYoshinori Hasegawa
Oct 22, 2002·American Journal of Physiology. Cell Physiology·Carole M LiedtkeXiangyun Wang
May 6, 2020·International Journal of Molecular Sciences·Margarida D AmaralInes Pankonien
Apr 6, 2007·American Journal of Physiology. Cell Physiology·Michael Auerbach, Carole M Liedtke
Jan 11, 2005·Ultramicroscopy·T BerdyyevaI Sokolov
Sep 2, 2014·Journal of Proteome Research·Mathieu Lavallée-AdamJohn R Yates
Sep 22, 2021·Biophysical Journal·Zhe GouChaouqi Misbah

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.