Evidence for direct transfer of tissue factor from monocytes to platelets in whole blood

Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis
Mikhail A SovershaevJohn-Bjarne Hansen

Abstract

Varying specificity of anti-tissue factor (anti-TF) antibodies gives rise to erroneous conclusions on TF positivity of platelets. Although monocytes are a well established source of TF in whole blood, there is no consensus whether platelets express or acquire TF from external sources. To test whether platelets can acquire TF expressed in monocytes, we studied a transfer of TF-yellow fluorescent protein (TF-YFP) from monocytes nucleofected with TF-YFP to platelets in a whole blood model. Platelets isolated from whole blood were found positive for TF when immunostained with anti-TF antibody from one supplier, whereas no platelet TF antigen was found in whole blood immunostained with anti-TF antibody from another supplier. Both antibodies recognized TF in monocytes. Platelets isolated from whole blood reconstituted with monocytes expressing TF-YFP fusion protein were found positive for TF-YFP only after stimulation with lipopolysaccharide (LPS). Taken together, TF protein could be transferred from monocytes upon stimulation with LPS.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B Osterud, S I Rapaport
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J N WilcoxD Gordon
Sep 1, 1982·The Journal of Clinical Investigation·G J Broze
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·P L GiesenY Nemerson
Feb 22, 2001·Biochemical and Biophysical Research Communications·A ZillmannB Engelmann
Mar 6, 2004·Journal of Thrombosis and Haemostasis : JTH·S NakamuraK Okamoto
Mar 6, 2004·Journal of Thrombosis and Haemostasis : JTH·B Østerud
Dec 18, 2004·Blood·Saulius ButenasKenneth G Mann
Apr 30, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Elena M EgorinaBjarne Østerud
Feb 16, 2006·Seminars in Thrombosis and Hemostasis·Bjarne Østerud, Eirik Bjørklid
Apr 26, 2006·Lupus·Ch López-PedreraM J Cuadrado
May 19, 2006·Journal of Thrombosis and Haemostasis : JTH·N MaugeriC Cerletti
Oct 25, 2006·The Journal of Experimental Medicine·Hansjörg SchwertzAndrew S Weyrich
Jan 4, 2007·Thrombosis and Haemostasis·Houria Mechiche, Philippe Nguyen
Mar 10, 2007·Blood·Olga PanesDiego Mezzano
Feb 13, 2010·Thrombosis Research·Bjarne Østerud
Aug 7, 2010·Blood·Beth A BouchardSaulius Butenas
Dec 4, 2010·Blood·Marina CameraElena Tremoli
May 14, 2011·Circulation Research·A Phillip Owens, Nigel Mackman
Jun 23, 2011·Journal of Thrombosis and Haemostasis : JTH·M G BasavarajJ-B Hansen

❮ Previous
Next ❯

Citations

Jan 1, 2012·Scientifica·Saulius Butenas
Jan 24, 2014·Thrombosis and Haemostasis·Florian Langer, Wolfram Ruf
Jun 5, 2013·Thrombosis Research·Nigel Mackman, Thomas Luther
Feb 14, 2018·Arteriosclerosis, Thrombosis, and Vascular Biology·Steven P Grover, Nigel Mackman
Nov 29, 2013·Journal of Advanced Nursing·John Paley
Jul 10, 2020·Translational Research : the Journal of Laboratory and Clinical Medicine·Ann S KimKeith R McCrae

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.