Evidence for distinct behaviour of phosphatidylcholine and sphingomyelin at the low density lipoprotein surface

Biochemical and Biophysical Research Communications
H C MurphyR A Iles

Abstract

This study demonstrates that the use of high field 1H NMR spectroscopy permits individual detection of phosphatidylcholine and sphingomyelin molecules at the surface of native low density lipoprotein (LDL) particles. Distinct behaviour was observed for the choline head group -N(CH3)3 resonances of these different phospholipids revealing preferential immobilisation for phosphatidylcholine. This suggests the existence of reversible and irreversible phosphatidylcholine-apolipoprotein B interactions and is consistent with microdomain formation at the surface monolayer of LDL. The novel resonance assignment and results show that 1H NMR can provide efficient and practical means for future studies on the structure and dynamics at the LDL surface.

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Citations

Jun 27, 2007·Biochemical and Biophysical Research Communications·Pasi SoininenMika Ala-Korpela
Jun 15, 2007·Chemistry and Physics of Lipids·Wenxian LanMaili Liu
Apr 25, 2006·Chemistry and Physics of Lipids·Marina KvederGreta Pifat
Oct 10, 2013·Biochemical and Biophysical Research Communications·Gérald GaibeletStéphane Orlowski
Jul 1, 2008·Journal of Lipid Research·Arnis Kuksis, Waldemar Pruzanski
May 29, 2000·The Journal of Biological Chemistry·J M HolopainenP K Kinnunen
Aug 11, 2000·The Journal of Biological Chemistry·S UrbanB Engelmann
Dec 26, 2006·Chemistry and Physics of Lipids·Elmar PrennerKarl Lohner

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