PMID: 2498838Apr 25, 1989Paper

Evidence for distinguishable transcripts of the putative testis determining gene (ZFY) and mapping of homologous cDNA sequences to chromosomes X,Y and 9

Nucleic Acids Research
N A AffaraM A Ferguson-Smith

Abstract

Oligonucleotide sequences based on the amino acid sequence of the putative testis determining gene ZFY have been used to isolate a 1.3 Kb Hind III Y genomic DNA fragment CMPXY1 and three human testis cDNA sequences (CMPXY2, CMPXY3 and CMPXY4). These sequences detect at least four potential exons on the Y (Y1, Y3, Y4 and Y5), three on the X (X1, X2 and X3) and three of autosomal origin (A1, A2 and A3) as determined by comparing the fragments detected by different clones. Analysis with subfragments of CMPXY4 shows that Y3 is unique to the Y and that Y4 and X1 are homologous. Y5 and X3 are detected by the same subfragment of CMPXY4. This is also the case for Y1, X2, A1, A2 and A3. Thus these exons may contain further regions of homology between the X, Y and an autosomal locus. The X-linked sequences all lie in Xp21.2-Xp22.1 and studies with XX males have placed the Y-linked sequences in distal Yp adjacent to the Y-autosomal homologous sequence GMGY3. We have confirmed these localizations by in situ hybridization with CMPXY4 and have shown additionally that the autosomal sequences of both the CMPXY4 sequence and GMGY3 map to 9p22-9pter. Restriction analysis demonstrates that CMPXY1/XY2/XY3 differ in sequence from CMPXY4 at three re...Continue Reading

References

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Citations

Nov 1, 1991·Human Genetics·G Müller, W Schempp
Jan 1, 1991·Mammalian Genome : Official Journal of the International Mammalian Genome Society·J H NadeauT H Roderick
Jan 1, 1992·Mammalian Genome : Official Journal of the International Mammalian Genome Society·J H NadeauT H Roderick
Nov 1, 1990·Developmental Biology·F A van der HoornS K Nordeen
Mar 25, 2000·Clinical Genetics·P VernoleP Grimaldi
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M S PalmerP N Goodfellow
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D A AdlerC M Disteche
Jun 1, 1993·Journal of Medical Genetics·C P BennettD Grant
Dec 29, 1989·Biochemical and Biophysical Research Communications·L M KalikinR P Erickson
Sep 1, 1991·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·N A Affara
Jul 1, 1997·Journal of Pediatric Endocrinology & Metabolism : JPEM·A RossodivitaF Colabucci

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