Abstract
Two unusual characteristics of some outer membrane proteins of Rhizobium leguminosarum are described. First, most of the major outer membrane proteins could only be visualized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis after lysozyme treatment of the isolated cell envelopes, suggesting a very strong, possibly covalent, interaction of these proteins with the peptidoglycan. These peptidoglycan-associated outer membrane proteins belonged to two distinct groups of immunologically related proteins, groups II and III, as defined by typing with monoclonal antibodies. As members of both groups of proteins could be radioactively labeled by growing cells in the presence of N-[3H]acetylglucosamine, we propose that variation in the apparent molecular weight of the antigens within each group is caused by varying numbers of peptidoglycan subunit residues on only two or three different outer membrane proteins. Second, group III outer membrane proteins, with masses of 35 to 46 kilodaltons, formed oligomers stabilized by divalent cations which resisted complete denaturation in 2% sodium dodecyl sulfate at 100 degrees C. Reconstitution experiments showed that of the divalent cations tested, Ca2+ and, to a lesser extent, Mn2+ an...Continue Reading
References
Oct 31, 1975·Biochimica Et Biophysica Acta·V Braun
Jun 7, 1979·Molecular & General Genetics : MGG·P M KlapwijkR A Schilperoort
Feb 1, 1979·European Journal of Biochemistry·E T Palva
Apr 1, 1979·Journal of Bacteriology·E T Palva, L L Randall
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Mar 17, 1977·Biochimica Et Biophysica Acta·B LugtenbergR Peters
Oct 15, 1975·FEBS Letters·B LugtenbergL van Alphen
Feb 1, 1989·Journal of Bacteriology·R de MaagdB J Lugtenberg
Jan 1, 1985·Gene·C Yanisch-PerronJ Messing
Feb 1, 1986·Journal of Bacteriology·L A de WegerB Lugtenberg
Jun 1, 1988·Journal of Bacteriology·J BerenguerM A de Pedro
Aug 1, 1988·Journal of Bacteriology·R de MaagdB J Lugtenberg
Apr 1, 1987·Journal of Bacteriology·J D RadolfM A Lovett
Sep 1, 1986·Journal of Bacteriology·R A de Maagd, B Lugtenberg
Feb 28, 1980·Biochimica Et Biophysica Acta·E T Palva
Jan 1, 1981·European Journal of Biochemistry·J Wensink, B Witholt
Jul 1, 1982·Plasmid·P J HooykaasR A Schilperoort
Sep 1, 1962·Journal of General Microbiology·J M VINCENT
Jul 13, 1963·Nature·J M VINCENT, B A HUMPHREY
Citations
Nov 1, 2017·Biomolecules·Daniel R SmithMatthew R Chapman
Feb 1, 1991·Oral Microbiology and Immunology·C MoutonL Lamy
Nov 15, 1996·FEMS Microbiology Letters·A CloeckaertN Vizcaíno
Sep 2, 2005·Molecular Plant-microbe Interactions : MPMI·Won-Seok KimHari B Krishnan
Jun 12, 2013·Journal of Bacteriology·Matthew B CrookJoel S Griffitts
Aug 20, 2011·Microbiology·Jun DongTanya E S Dahms
Jan 1, 1992·Journal of Bacteriology·R A de MaagdB J Lugtenberg
Nov 5, 2002·Veterinary Microbiology·Axel CloeckaertPhilip H Elzer
Aug 1, 1993·Journal of Bacteriology·Y R ThorstensonP C Zambryski
Sep 1, 1995·Journal of Bacteriology·H P RoestB J Lugtenberg
Aug 1, 1994·Trends in Microbiology·E L Kannenberg, N J Brewin