Evidence for effect of GM1 on opioid peptide conformation: NMR study on leucine enkephalin in ganglioside-containing isotropic phospholipid bicelles

Langmuir : the ACS Journal of Surfaces and Colloids
Anindita Gayen, Chaitali Mukhopadhyay

Abstract

Enkephalins are endogenous neuropeptides that have opioid-like activities and compete with morphines for the receptor binding. The binding of these neuropeptides to membrane appears crucial since enkephalins interact with the nerve cell membranes to achieve bioactive conformations that fit onto multiple receptor sites (micro, delta, and kappa). Using NMR spectroscopy, we have determined the solution structure of the small opiate pentapeptide leucine enkephalin in the presence of isotropic phospholipid bicelles: phosphocholine bicelles (DMPC:CHAPS 1:4) and phosphocholine bicelles doped with ganglioside GM1 (DMPC:CHAPS:GM1 1:4:0.3). Bicelles containing GM1 were found to interact strongly with leucine enkephalin, whereas a somewhat weaker interaction was observed in the case of bicelles without GM1. Structure calculation from torsion angles, chemical shifts, and NOE-based distance constraints explored that the peptide could flexibly switch between several mu- and delta-selective conformations in both the bicelles though micro-selective conformations turned out to be geometrically preferred in each bicellar system. A detailed analysis of the structures presented supports the variance over the singly associated conformation of enkep...Continue Reading

References

Jul 1, 1991·Biopolymers·G V NikiforovichC A Gehrig
Oct 7, 1986·Biochemistry·R Schwyzer
Nov 1, 1983·Journal of Neurochemistry·R W McLawhonG Dawson
May 6, 1994·Chemistry and Physics of Lipids·S SonninoB Venerando
Feb 1, 1996·Journal of Molecular Graphics·R KoradiK Wüthrich
Dec 1, 1996·Journal of Biomolecular NMR·R A LaskowskiJ M Thornton
May 1, 1997·Biophysical Journal·D van der Spoel, H J Berendsen
Dec 3, 1998·Experimental Brain Research·H Steiner, C R Gerfen
Feb 13, 2001·Biophysical Journal·J A WhilesR R Vold
Apr 25, 2001·Journal of Magnetic Resonance·C D Schwieters, G M Clore
Mar 28, 2002·Biophysical Journal·Min-yi Shen My, Karl F Freed
Jul 13, 2002·Biopolymers·Mahalaxmi Aburi, Paul E Smith
Feb 5, 2003·Journal of Magnetic Resonance·Charles D SchwietersG Marius Clore
Dec 4, 2003·Biopolymers·Chiradip Chatterjee, Chaitali Mukhopadhyay
Apr 14, 2005·Journal of the American Chemical Society·Muthu DhanasekaranRobin Polt
Jul 22, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Thomas B CardonGary A Lorigan
Apr 8, 2006·Magnetic Resonance in Chemistry : MRC·Mathias Nilsson, Gareth A Morris
May 24, 2006·Biochimica Et Biophysica Acta·Ulrich H N DürrAyyalusamy Ramamoorthy
Sep 5, 2006·Biochimica Et Biophysica Acta·August AnderssonLena Mäler
Sep 19, 2006·Biochimica Et Biophysica Acta·Vishnu DhopleAyyalusamy Ramamoorthy
Nov 7, 2006·Journal of Magnetic Resonance·Sergey V DvinskikhAyyalusamy Ramamoorthy
Jan 25, 2007·Journal of the American Chemical Society·Sergey V DvinskikhAyyalusamy Ramamoorthy
Feb 8, 2007·Journal of the American Chemical Society·Sébastien F PogetMark E Girvin
May 10, 2007·Journal of the American Chemical Society·Ulrich H N DürrAyyalusamy Ramamoorthy
Nov 13, 2007·Journal of the American Chemical Society·Nobuaki MatsumoriMichio Murata

❮ Previous
Next ❯

Citations

Jul 16, 2013·Biophysical Chemistry·Ummul Liha Khatun, Chaitali Mukhopadhyay
Sep 8, 2010·Natural Product Reports·Nobuaki Matsumori, Michio Murata
Jul 25, 2012·Biophysical Chemistry·Ummul Liha KhatunChaitali Mukhopadhyay
Mar 1, 2012·Chemistry and Physics of Lipids·Burkhard Bechinger, Evgeniy S Salnikov
Oct 2, 2009·Peptides·Richard J Bodnar
Apr 12, 2011·Biochimica Et Biophysica Acta·Dror E WarschawskiIsabelle Marcotte
Oct 13, 2010·Biochimica Et Biophysica Acta·Anindita GayenChaitali Mukhopadhyay
Sep 8, 2009·Biochimica Et Biophysica Acta·Jacques Fantini, Francisco J Barrantes
Jun 11, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·K S MineevA S Arseniev
Jan 9, 2013·Chemical Communications : Chem Comm·Takumi YamaguchiKoichi Kato
Jun 4, 2021·Progress in Nuclear Magnetic Resonance Spectroscopy·Pedro Lameiras, Jean-Marc Nuzillard
Mar 2, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Moutusi Manna, Chaitali Mukhopadhyay
Aug 28, 2012·Chemical Reviews·Ulrich H N DürrAyyalusamy Ramamoorthy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.