Evidence for Environmental-Human Microbiota Transfer at a Manufacturing Facility with Novel Work-related Respiratory Disease.

American Journal of Respiratory and Critical Care Medicine
Benjamin G WuLeopoldo N Segal

Abstract

Rationale: Workers' exposure to metalworking fluid (MWF) has been associated with respiratory disease.Objectives: As part of a public health investigation of a manufacturing facility, we performed a cross-sectional study using paired environmental and human sampling to evaluate the cross-pollination of microbes between the environment and the host and possible effects on lung pathology present among workers.Methods: Workplace environmental microbiota were evaluated in air and MWF samples. Human microbiota were evaluated in lung tissue samples from workers with respiratory symptoms found to have lymphocytic bronchiolitis and alveolar ductitis with B-cell follicles and emphysema, in lung tissue samples from control subjects, and in skin, nasal, and oral samples from 302 workers from different areas of the facility. In vitro effects of MWF exposure on murine B cells were assessed.Measurements and Main Results: An increased similarity of microbial composition was found between MWF samples and lung tissue samples of case workers compared with control subjects. Among workers in different locations within the facility, those that worked in the machine shop area had skin, nasal, and oral microbiota more closely related to the microbiot...Continue Reading

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Citations

Aug 22, 2020·American Journal of Respiratory and Critical Care Medicine·Michael P Combs, Robert P Dickson
Jan 5, 2021·Current Opinion in Allergy and Clinical Immunology·Randall J NettGeorge R Grimes

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Datasets Mentioned

BETA
PRJNA635409
PRJNA635410

Methods Mentioned

BETA
biopsies
biopsy
amplicon sequencing
flow cytometry
fluorescence-activated cell-sorting

Software Mentioned

Quantitative Insights into Microbial Ecology
global
DESeq2
PERMANOVA
VSEARCH
Decontam
R
Basic Local Alignment Search Tool
UCLUST
usearch

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