Sep 1, 1978

Evidence for existence of type A MAO in mitochondria from human placenta (author's transl)

Nihon yakurigaku zasshi. Folia pharmacologica Japonica
R Kikuchi, H Kinemuchi

Abstract

The existence of type A and B MAO in mitochondria from human placenta was investigated on the basis of inhibitions by selective MAO inhibitors, such as clorgyline (type A inhibitor) and pargyline and deprenyl (type B inhibitors) with serotonin (substrate for type A MAO), tyramine (substrate for both types of MAO) and beta-phenylethylamine (substrate for type B MAO) as substrates and the results were compared with those obtained with MAO in rat liver. The rates of serotonin, beta-phenylethylamine and benzylamine oxidations by placental MAO were approximately 191, 12 and 48% to those of rat liver MAO, respectively. Placental MAO was more sensitive to tryptic digestion than the enzyme in rat liver. Both MAO's could be separated into two fractions by sucrose density gradient centrifugation, but the two types could not be distinguished when inhibitor sensitivity and substrate specificity experiments were carried out. Placental MAO activity was inhibited by low concentrations of type A inhibitor and was relatively insensitive to those of type B. Simple sigmoidal and identical inhibition curves with various concentrations of either type A or type B inhibitors were obtained with these substrates. These findings suggest that mitochondri...Continue Reading

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Mentioned in this Paper

Benzylamine
Centrifugation, Density Gradient
Phenethylamine
Placenta Specimen
Serotonin Measurement
Serotonin
Placenta
Mitochondria, Liver
Monoamine Oxidase Inhibitors
Oxidation

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