Evidence for gene duplication in the voltage-gated sodium channel gene of Aedes aegypti

Evolution, Medicine, and Public Health
Ademir J MartinsAlexandre Afranio Peixoto

Abstract

Mutations in the voltage-gated sodium channel gene (NaV), known as kdr mutations, are associated with pyrethroid and DDT insecticide resistance in a number of species. In the mosquito dengue vector Aedes aegypti, besides kdr, other polymorphisms allowed grouping AaNaV sequences as type 'A' or 'B'. Here, we point a series of evidences that these polymorphisms are actually involved in a gene duplication event. Four series of methods were employed: (i) genotypying, with allele-specific PCR (AS-PCR), of two AaNaV sites that can harbor kdr mutations (Ile1011Met and Val1016Ile), (ii) cloning and sequencing of part of the AaNaV gene, (iii) crosses with specific lineages and analysis of the offspring genotypes and (iv) copy number variation assays, with TaqMan quantitative real-time PCR. kdr mutations in 1011 and 1016 sites were present only in type 'A' sequences, but never in the same haplotype. In addition, although the 1011Met-mutant allele is widely disseminated, no homozygous (1011Met/Met) was detected. Sequencing revealed three distinct haplotypes in some individuals, raising the hypothesis of gene duplication, which was supported by the genotype frequencies in the offspring of specific crosses. Furthermore, it was estimated that...Continue Reading

References

Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M Kimura, J L King
May 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·D T SuzukiR Williamson
Nov 1, 1996·Journal of Neurochemistry·D BourguetM Arpagaus
Feb 18, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·M RaymondN Pasteur
Apr 27, 2002·Genetical Research·Claire BerticatChristine Chevillon
Oct 5, 2002·Science·Hilary RansonRené Feyereisen
Jul 11, 2003·Nature·Balázs PappLaurence D Hurst
Oct 7, 2004·Genetical Research·Claire BerticatMichel Raymond
Feb 16, 2006·The Biochemical Journal·Andrias O O'ReillyTg Emyr Davies
May 10, 2007·IUBMB Life·Tg Emyr DaviesMartin S Williamson
Dec 12, 2007·Insect Biochemistry and Molecular Biology·Clare StrodeHilary Ranson
Apr 3, 2008·Pest Management Science·David M Soderlund
May 6, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Haoues AloutMylène Weill
Jun 19, 2008·Pest Management Science·Tg Emyr DaviesMartin S Williamson
Nov 19, 2008·Nature Reviews. Genetics·Gavin C Conant, Kenneth H Wolfe
Mar 11, 2009·Memórias do Instituto Oswaldo Cruz·Thiago Affonso BelinatoDenise Valle
Sep 9, 2009·Tropical Medicine & International Health : TM & IH·Ademir J MartinsDenise Valle
Oct 6, 2009·Insect Biochemistry and Molecular Biology·Bénédicte MoignotChristian Legros
Oct 16, 2009·PLoS Neglected Tropical Diseases·Gustavo Ponce GarcíaWilliam C Black IV
Jul 6, 2010·Insect Biochemistry and Molecular Biology·Kentaro ItokawaTakashi Tomita
Aug 5, 2010·The American Journal of Tropical Medicine and Hygiene·Angela F HarrisHilary Ranson
Jan 14, 2011·Parasites & Vectors·Estelita Pereira LimaMaria Alice V de Melo Santos
Mar 23, 2011·Biochemical and Biophysical Research Communications·Qiang XuNannan Liu
Mar 31, 2011·ILAR Journal·Ana Tereza Pinto FilipeckiMárcia de Oliveira Teixeira
May 4, 2011·Pest Management Science·Chris Bass, Linda M Field
Feb 11, 2012·Insect Biochemistry and Molecular Biology·Ying-Jun CuiChuan-Xi Zhang
Jun 6, 2012·Memórias do Instituto Oswaldo Cruz·Isabela Reis MontellaDenise Valle

Citations

Jan 1, 1994·Parasitology Today·J A Urbina
Jun 19, 2015·Biochemical and Biophysical Research Communications·Cara LeeMichael D Carrithers
Jun 6, 2015·Pesticide Biochemistry and Physiology·René FeyereisenThomas Van Leeuwen
Nov 21, 2015·PLoS Neglected Tropical Diseases·Isabelle DusfourClare Strode
Nov 7, 2016·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Xuan-Zhao JiangJin-Jun Wang
Jul 28, 2019·Genome Research·Eric R LucasAnopheles gambiae 1000 Genomes Consortium
Apr 17, 2020·PLoS Neglected Tropical Diseases·Luciano Veiga CosmeAdemir J Martins
May 8, 2020·Archives of Insect Biochemistry and Physiology·Mengli ChenKe Dong
Aug 9, 2020·Scientific Reports·Monique Melo CostaAdemir J Martins
Nov 8, 2018·BioMed Research International·Mariana Rocha DavidRafael Maciel-de-Freitas