PMID: 6970226Mar 1, 1981Paper

Evidence for homologues of the murine I-A and I-E loci in the rat MHC

The Journal of Immunology : Official Journal of the American Association of Immunologists
N Shinohara, D H Sachs

Abstract

Various anti-Ia mouse alloantisera were examined for cross-reactivity with rat lymphocytes. Many of them were found to cross-react with rat spleen cells with anti-Ia-type (40 to 60%) killing. Likewise, mouse antisera raised against rat lymphocytes cross-reacted with mouse cells, and some of these reactions could be mapped within the I-A subregion and some within the I-E/C subregion of the mouse H-2 complex. Using such cross-reactive mouse alloantisera and a defined anti-MHC rat alloantiserum (BN anti-LEW), SDS-PAGE analyses were performed on an NP-40 extract of internally labeled LEW antigen. The cross-reactive mouse alloantisera precipitated Ia-like glycoproteins (35,000 and 28,000 m.w.), which could be completely removed by pretreatment with the rat anti-MHC antiserum. The cross-reactive anti-I-A and anti-I-E/C mouse antibodies were found to react with 2 different classes of rat Ia molecules by sequential precipitation analysis. On the basis of the specificity of the mouse alloantibodies, one Ia molecule appears to be a homologue of the murine I-A antigens and the other a homologue of the murine I-E antigens. Preabsorption of LEW antigen with cross-reactive anti-I-A and anti-I-E/C mouse alloantibodies together did not leave a...Continue Reading

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