Evidence for homoploid speciation in Phytophthora alni supports taxonomic reclassification in this species complex

Fungal Genetics and Biology : FG & B
C HussonB Marçais

Abstract

Alder decline has been a problem along European watercourses since the early 1990s. Hybridization was identified as the main cause of this emerging disease. Indeed, the causal agent, a soil-borne pathogen named Phytophthora alni subsp. alni (Paa) is the result of interspecific hybridization between two taxa, Phytophthora alni subsp. multiformis (Pam) and Phytophthora alni subsp. uniformis (Pau), initially identified as subspecies of Paa. The aim of this work was to characterize the ploidy level within the P. alni complex that is presently poorly understood. For that, we used two complementary approaches for a set of 31 isolates of Paa, Pam and Pau: (i) quantification of allele copy number of three single-copy nuclear genes using allele-specific real-time PCR and (ii) comparison of the genome size estimated by flow cytometry. Relative quantification of alleles of the three single-copy genes showed that the copy number of a given allele in Paa was systematically half that of its parents Pau or Pam. Moreover, DNA content estimated by flow cytometry in Paa was equal to half the sum of those in Pam and Pau. Our results therefore suggest that the hybrid Paa is an allotriploid species, containing half of the genome of each of its pare...Continue Reading

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Citations

Apr 27, 2016·Current Opinion in Microbiology·Jasper Rl DepotterBart Phj Thomma
Oct 19, 2016·Genomics Data·Nicolas FeauRichard C Hamelin
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Dec 16, 2017·IMA Fungus·Xiao YangChuanxue Hong
Feb 2, 2020·Applied Microbiology and Biotechnology·Nicola LuchiAlberto Santini

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