Evidence for Intracellular and Extracellular Dimethylsulfoniopropionate (DMSP) Lyases and DMSP Uptake Sites in Two Species of Marine Bacteria.

Applied and Environmental Microbiology
D C YochK S Rabinowitz

Abstract

The kinetics of dimethylsulfoniopropionate (DMSP) uptake and dimethylsulfide (DMS) production from DMSP in two bacterial species, Alcaligenes sp. strain M3A, an isolate from estuarine surface sediments, and Pseudomonas doudoroffii, from seawater, were investigated. In Alcaligenes cells induced for DMSP lyase (DL) activity, DMS production occurred without DMSP uptake. In DL-induced suspensions of P. doudoroffii, uptake of DMSP preceded the production of DMS, indicating an intracellular location of DL; intracellular DMSP levels reached ca. 7 mM. DMSP uptake rates in noninduced cells showed saturation at three concentrations (K(inft) [transport] values, 3.4, 127, and 500 (mu)M). In DL-induced cells of P. doudoroffii, DMSP uptake rates increased ca. threefold (V(infmax), 0.022 versus 0.065 (mu)mol of DMSP taken up min(sup-1) mg of cell protein(sup-1)), suggesting that the uptake binding proteins were inducible. DMSP uptake and DL activity in P. doudoroffii were both inhibited by CN(sup-), 2,4-dinitrophenol, and membrane-impermeable thiol-binding reagents, further indicating active uptake of DMSP by cell surface components. The respiratory inhibitors had limited or no effect on DL activity by the Alcaligenes sp. Of the structural an...Continue Reading

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Citations

Jun 9, 2004·Applied and Environmental Microbiology·Todd R Miller, Robert Belas
Jan 3, 2015·Organic & Biomolecular Chemistry·Jeroen S DickschatChristian A Citron
Jul 27, 2007·Natural Product Reports·Stefan Schulz, Jeroen S Dickschat
Sep 15, 2007·Nature Reviews. Microbiology·Farooq Azam, Francesca Malfatti
Jun 1, 2012·Journal of Phycology·Pilar García-Jiménez, Rafael R Robaina
Nov 25, 2005·Photosynthesis Research·Alessandra NoriciMario Giordano
Dec 14, 2005·Applied and Environmental Microbiology·M K Bacic, D C Yoch
Dec 14, 2005·Applied and Environmental Microbiology·M K BacicD C Yoch

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