Evidence for intragenic recombination and selective sweep in an effector gene of Phytophthora infestans

Evolutionary Applications
Li-Na YangJiasui Zhan

Abstract

Effectors, a group of small proteins secreted by pathogens, play a critical role in the antagonistic interaction between plant hosts and pathogens through their dual functions in regulating host immune systems and pathogen infection capability. In this study, evolution in effector genes was investigated through population genetic analysis of Avr3a sequences generated from 96 Phytophthora infestans isolates collected from six locations representing a range of thermal variation and cropping systems in China. We found high genetic variation in the Avr3a gene resulting from diverse mechanisms extending beyond point mutations, frameshift, and defeated start and stop codons to intragenic recombination. A total of 51 nucleotide haplotypes encoding 38 amino acid isoforms were detected in the 96 full sequences with nucleotide diversity in the pathogen populations ranging from 0.007 to 0.023 (mean = 0.017). Although haplotype and nucleotide diversity were high, the effector gene was dominated by only three haplotypes. Evidence for a selective sweep was provided by (i) the population genetic differentiation (GST) of haplotypes being lower than the population differentiation (FST) of SSR marker loci; and (ii) negative values of Tajima's D ...Continue Reading

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Citations

Jun 15, 2021·Frontiers in Microbiology·Abdul WaheedJiasui Zhan
Feb 2, 2021·Molecular Plant-microbe Interactions : MPMI·Soledad SacristánSebastian Eves-van den Akker
Oct 10, 2021·Molecular Plant-microbe Interactions : MPMI

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Datasets Mentioned

BETA
MH043150
MH043151
MH043152
MH043153
MH043154
MH043155
MH043156
MH043160

Methods Mentioned

BETA
dissection
PCR
electrophoresis

Software Mentioned

MEME
DnaSP
MrModelTest
RDP
TRACER
MEGA5
SiScan
IFEL
RDP4
MrBayes

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