PMID: 8604133Mar 1, 1996Paper

Evidence for involvement of Escherichia coli genes pmbA, csrA and a previously unrecognized gene tldD, in the control of DNA gyrase by letD (ccdB) of sex factor F

Journal of Molecular Biology
N MurayamaT Miki

Abstract

The letA (ccdA) and letD (ccdB) genes of the F plasmid, located just outside the sequence essential for replication, contribute to stable maintenance of the plasmid in Escherichia coli cells. The letD gene product acts to inhibit partitioning of chromosomal DNA and cell division by inhibiting DNA gyrase activity, whereas the letA gene product acts to reverse the inhibitory activity of the letD gene product. To identify the host factor(s) involved in this process, we analyzed the mutants that escaped letD expression and their suppressor, and found that the three E. coli genes tldD, tldE and zfiA participate in the process, in addition to the groE genes we reported previously. The tldD and tldE mutations made cells tolerant for letD expression, as did groES mutations, while the mutation in the zfiA gene made tldD, tldE and groES mutants LetD sensitive. We hypothesize that these gene products are factors that modulate activity of DNA gyrase along with the letD gene product; the zfiA gene product acts to inhibit interaction between the LetD protein and the A subunit of DNA gyrase, while the tldD, tldE and groE gene products act to suppress the inhibitory activity of the zfiA gene product. The tldD, tldE, and zfiA genes are located ...Continue Reading

Citations

Feb 6, 2008·Environmental Microbiology·Evelyne KrinAntoine Danchin
Aug 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R J LewisD B Wigley
Nov 5, 1999·Annual Review of Microbiology·H Engelberg-Kulka, G Glaser
Feb 26, 2004·FEMS Microbiology Letters·Rose-May DelrueXavier De Bolle
Aug 26, 1998·Trends in Microbiology·M CouturierL Van Melderen
Sep 18, 1997·Microbiology and Molecular Biology Reviews : MMBR·K Drlica, X Zhao
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn
Jun 1, 2007·Journal of Proteome Research·Jens GlaeserGabriele Klug

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