PMID: 6402782Mar 1, 1983Paper

Evidence for long-lived mRNA during fruiting body formation in myxococcus xanthus

Proceedings of the National Academy of Sciences of the United States of America
D R Nelson, D R Zusman

Abstract

Half-lives of Myxococcus xanthus mRNA were determined by inhibiting RNA polymerase with rifampin and then measuring the rate of [35S]methionine incorporation into protein. Vegetative cells resuspended in clone fruiting liquid culture showed an average mRNA half-life of approximately 3.5 min. Developmental cells (24 or 48 hr) exhibited biphasic decay curves with apparent mRNA half-lives of approximately 3.5 min and 20-30 min. The more-stable mRNA species accounted for about 30% of all mRNA present in 24-hr cells and as much as 40% of all mRNA of 48-hr cells. Analysis of the 35S-labeled proteins by NaDodSO4/polyacrylamide gel electrophoresis showed that only 5-10 major polypeptides are synthesized after 30 min of rifampin treatment. One of these is protein S, the spore surface coat protein, because immunoprecipitation of the 35S-labeled proteins with antisera specific for protein S showed continued synthesis of protein S in the presence of rifampin. The half-life for its mRNA was calculated to be 15-30 min. RNA from vegetative and developing cells was pulse labeled with 32PO4 followed by rifampin treatment. Analysis of the labeled RNA on 3% NaDodSO4/polyacrylamide gels showed 5-10 long-lived mRNA bands in developing cells. These ...Continue Reading

References

Jun 1, 1978·Developmental Biology·D C HagenD Kaiser
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Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M InouyeD R Zusman
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Citations

Sep 1, 1986·Developmental Biology·L KroosD Kaiser
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J M RomeoD R Zusman
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·O ResnekovA von Gabain
Jun 5, 1984·Journal of Molecular Biology·J S DownardD R Zusman
May 23, 2015·FEMS Microbiology Reviews·Mark A LeverBo Barker Jørgensen
May 1, 2015·PLoS Computational Biology·Albertas JanuleviciusCristian Picioreanu
Nov 1, 1993·Physical Review. B, Condensed Matter·R BradaW I Glaberson
Oct 1, 1992·Molecular Microbiology·J M Romeo, D R Zusman
Jan 1, 1987·Critical Reviews in Microbiology·L J Shimkets
Jul 1, 1997·Microbiology·David R NelsonStaffan Kjelleberg
Mar 1, 1984·Journal of Bacteriology·P K Majumdar, B A McFadden
Dec 1, 1986·Journal of Bacteriology·D R Nelson, K P Killeen
Mar 1, 1985·Journal of Bacteriology·J S Downard, D R Zusman
Dec 1, 1990·Microbiological Reviews·L J Shimkets

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