Feb 14, 2006

Evidence for motoneuron lineage-specific regulation of Olig2 in the vertebrate neural tube

Developmental Biology
Tao SunD H Rowitch

Abstract

Within the motoneuron precursor (pMN) domain of the developing spinal cord, the bHLH transcription factor, Olig2, plays critical roles in pattern formation and the generation of motor neuron and oligodendrocyte precursors. How are the multiple functions of Olig2 regulated? We have isolated a large BAC clone encompassing the human OLIG2 locus that rescues motor neuron and oligodendrocyte development but not normal pattern formation in Olig2(-/-) embryos. Within the BAC clone, we identified a conserved 3.6 kb enhancer sub-region that directs reporter expression specifically in the motor neuron lineage but not oligodendrocyte lineage in vivo. Our findings indicate complex regulation of Olig2 by stage- and lineage-specific regulatory elements. They further suggest that transcriptional regulation of Olig2 is involved in segregation of pMN neuroblasts.

  • References32
  • Citations11

Citations

Mentioned in this Paper

Vertebrates
Embryo
Galactosidase Activity
Transcriptional Regulation
Neural Tube
Equine Laminitis
Untranslated Regions
Immunohistochemistry
AT 17
Genome

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