Evidence for multiple cycles of strand invasion during repair of double-strand gaps in Drosophila

Genetics
Mitch McVeyJeff Sekelsky

Abstract

DNA double-strand breaks (DSBs), a major source of genome instability, are often repaired through homologous recombination pathways. Models for these pathways have been proposed, but the precise mechanisms and the rules governing their use remain unclear. In Drosophila, the synthesis-dependent strand annealing (SDSA) model can explain most DSB repair. To investigate SDSA, we induced DSBs by excision of a P element from the male X chromosome, which produces a 14-kb gap relative to the sister chromatid. In wild-type males, repair synthesis tracts are usually long, resulting in frequent restoration of the P element. However, repair synthesis is often incomplete, resulting in internally deleted P elements. We examined the effects of mutations in spn-A, which encodes the Drosophila Rad51 ortholog. As expected, there is little or no repair synthesis in homozygous spn-A mutants after P excision. However, heterozygosity for spn-A mutations also resulted in dramatic reductions in the lengths of repair synthesis tracts. These findings support a model in which repair DNA synthesis is not highly processive. We discuss a model wherein repair of a double-strand gap requires multiple cycles of strand invasion, synthesis, and dissociation of t...Continue Reading

References

Sep 1, 1993·Genetics·G B GloorW R Engels
Aug 15, 1997·Genes & Development·E L Beall, D C Rio
Oct 29, 1997·Molecular and Cellular Biology·E Rubin, A A Levy
Apr 7, 1998·Molecular and Cellular Biology·F PâquesJ E Haber
May 29, 2000·Trends in Genetics : TIG·J E Haber
Mar 10, 2001·Nature·E S LanderUNKNOWN International Human Genome Sequencing Consortium
Nov 13, 2002·Mutation Research·Larry H Thompson, David Schild
Nov 28, 2002·Molecular Cell·Jachen A SolingerWolf-Dietrich Heyer
Aug 13, 2003·The Journal of Biological Chemistry·Patrick SungMichael G Sehorn
Nov 1, 2003·The EMBO Journal·Eric Staeva-VieiraRuth Lehmann

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Citations

Jul 15, 2009·Nature Reviews. Genetics·P J HastingsGrzegorz Ira
Feb 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Wade M HicksJames E Haber
Nov 17, 2007·Nucleic Acids Research·Robert M Brosh, Vilhelm A Bohr
Sep 20, 2012·Nucleic Acids Research·Aaron Mendez-BermudezNicola J Royle
Mar 29, 2013·Nucleic Acids Research·Jessica L SneedenWolf-Dietrich Heyer
Aug 30, 2007·Genome Research·Anna-Sophie Fiston-LavierHadi Quesneville
Sep 7, 2006·Molecular and Cellular Biology·Ganesh NagarajuRalph Scully
Feb 16, 2010·Molecular and Cellular Biology·Jeannine R Larocque, Maria Jasin
Nov 9, 2007·PLoS Biology·Melissa S McMahillDouglas K Bishop
Jun 23, 2006·PLoS Genetics·Peter L Houston, James R Broach
Apr 17, 2007·PLoS Genetics·Dena M Johnson-SchlitzWilliam R Engels
Nov 22, 2005·Genetics·Christine R PrestonWilliam R Engels
May 17, 2006·Genetics·Dena M Johnson-Schlitz, William R Engels
Dec 21, 2006·Genetics·Hajime TakeuchiDieter Egli
Jul 31, 2007·Genetics·Debbie S Wei, Yikang S Rong
Mar 20, 2014·Seminars in Cell & Developmental Biology·Marco MilánMariana Muzzopappa
Nov 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Frank HartungHolger Puchta
Oct 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Mitch McVeyJeff J Sekelsky
Feb 17, 2010·Methods : a Companion to Methods in Enzymology·Sierra ColavitoPatrick Sung
Apr 28, 2009·DNA Repair·Sushmita MukherjeeJeff Sekelsky
Oct 9, 2015·Wiley Interdisciplinary Reviews. Developmental Biology·Koen J T VenkenKristi L Hoffman
May 8, 2014·Nucleic Acids Research·Alekos SimoniAndrea Crisanti
Jun 18, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Michael Leffak
Apr 6, 2007·Nature·Catherine E SmithLorraine S Symington
Mar 31, 2018·The Journal of Biological Chemistry·William Douglass WrightWolf-Dietrich Heyer

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