PMID: 9173880Apr 1, 1997Paper

Evidence for multiple forms of biotin holocarboxylase synthetase in pea (Pisum sativum) and in Arabidopsis thaliana: subcellular fractionation studies and isolation of a cDNA clone

The Biochemical Journal
G TissotC Alban

Abstract

The intracellular compartmentation of biotin holocarboxylase synthetase has been investigated in pea (Pisum sativum) leaves, by isolation of organelles and fractionation of protoplasts. Enzyme activity was mainly located in cytosol (approx. 90% of total cellular activity). Significant activity was also identified in the soluble phase of both mitochondria and chloroplasts. Two enzyme forms were separated by anion-exchange chromatography. The major form was found to be specific for the cytosol compartment, whereas the minor form was present in mitochondria as well as in chloroplasts. We also report the isolation and DNA sequence of a cDNA encoding an Arabidopsis thaliana biotin holocarboxylase synthetase. This cDNA was isolated by functional complementation of a conditional lethal Escherichia coli birA (biotin ligase gene, which regulates biotin synthesis) mutant. This indicated that the recombinant plant protein was able to biotinylate specifically an essential apoprotein substrate in the bacterial host, that is a subunit of acetyl-CoA carboxylase called biotin carboxyl carrier protein. The full-length nucleotide sequence (1534 bp) encodes a protein of 367 amino acid residues with a molecular mass of 41172 Da and shows specific ...Continue Reading

Citations

Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Claude AlbanRoland Douce
Apr 22, 1999·Protein Science : a Publication of the Protein Society·D BeckettP J Schatz
Nov 21, 2001·Protein Science : a Publication of the Protein Society·A Chapman-SmithJ C Wallace
Sep 19, 2002·The Journal of Biological Chemistry·Sanja RojeAndrew D Hanson
Jan 11, 2002·The Journal of Biological Chemistry·Laurence DenisClaude Alban
Feb 16, 2002·Protein Science : a Publication of the Protein Society·Keehwan KwonDorothy Beckett
Apr 15, 2004·Nature Reviews. Microbiology·Stuart A RalphGeoffrey I McFadden
Apr 2, 2003·The Journal of Biological Chemistry·Eoin P QuinlivanAndrew D Hanson
Feb 28, 2018·Proceedings of the National Academy of Sciences of the United States of America·Teegan A Dellibovi-RaghebSean T Prigge
Apr 28, 2007·Photosynthesis Research·Fabrice RébeilléRoland Douce
Dec 18, 2004·Plant Molecular Biology·Claus-Peter WitteTina Romeis
Sep 7, 2000·Protein Science : a Publication of the Protein Society·K Kwon, D Beckett
Mar 4, 1999·The Journal of Nutrition·A Chapman-Smith, J E Cronan
Jan 13, 2009·Plant Physiology·Pascal RippertMichel Matringe
Oct 6, 2000·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·C Alban
Jun 5, 2003·Archives of Biochemistry and Biophysics·Basil J NikolauEve Syrkin Wurtele
Aug 29, 2006·Biochemical and Biophysical Research Communications·Thurid BoetelAndreas Frey

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