Evidence for multiple molecular forms of yeast beta-glucosidase in a hybrid yeast.

Journal of Bacteriology
L W Fleming, J D Duerksen

Abstract

A mixture of beta-glucosidases from Saccharomyces fragilis (Y-18) and S. dobzhanskii (Y-19) eluted from diethylaminoethyl cellulose in two peaks, whereas the enzyme from a hybrid, S. fragilis x S. dobzhanskii (Y-42), eluted in a single broad peak. The highest Y-42 activity fractions eluted at a sodium chloride molarity which was intermediate to the molarities at which most of the Y-18 and Y-19 activity was eluted. In cellulose polyacetate strips, Y-42 enzyme migrated as a diffuse band which spanned the distances migrated by the enzymes from the parent yeast strains. Antisera against either Y-18 or Y-19 enzyme precipitated 80 to 90% of Y-42 enzyme activity. When Y-42 enzyme was dissociated by heat or urea and reacted with parental antiserum, a concomitant increase in the opposite parental activity was demonstrable in both precipitation and complement-fixation (CF) tests. Urea-dissociated beta-glucosidases were resolvable by sucrose-gradient centrifugation into multiple bands displaying specific CF activity. When the enzymes were exposed to 4 m urea for 12 min, particles of approximately 110,000 molecular weight were obtained. By extending the exposure time to 40 min, and incorporating 0.5 m urea in the gradients, smaller particl...Continue Reading

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Citations

Oct 15, 1968·Experientia·C L Markert, G S Whitt
Aug 15, 1988·European Journal of Biochemistry·E Yagüe, M P Estévez
Apr 1, 1972·Journal of Bacteriology·M Tingle, H O Halvorson
Oct 1, 1968·Journal of Bacteriology·G L Marchin, J D Duerksen
Oct 1, 1968·Journal of Bacteriology·G L Marchin, J D Duerksen
Jan 1, 1967·Journal of Bacteriology·L W Fleming, J D Duerksen
Mar 1, 1991·Applied and Environmental Microbiology·S N Freer
Dec 1, 1969·Journal of Bacteriology·Y W Han, V R Srinivasan
Feb 1, 1970·Journal of Bacteriology·J N Bicknell, H C Douglas
Aug 15, 1969·Science·J M Mitchison

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