Evidence for multiple open and inactivated states of the hKv1.5 delayed rectifier

Biophysical Journal
T C Rich, Dirk J Snyders

Abstract

The kinetic properties of hKv1.5, a Shaker-related cardiac delayed rectifier, expressed in Ltk- cells were studied. hKv1.5 currents elicited by membrane depolarizations exhibited a delay followed by biphasic activation. The biphasic activation remained after 5-s prepulses to membrane potentials between -80 and -30 mV; however, the relative amplitude of the slow component increased as the prepulse potential approached the threshold of channel activation, suggesting that the second component did not reflect activation from a hesitant state. The decay of tail currents at potentials between -80 and -30 mV was adequately described with a biexponential. The time course of deactivation slowed as the duration of the depolarizing pulse increased. This was due to a relative increase in the slowly decaying component, despite similar initial amplitudes reflecting a similar open probability after 50- and 500-ms prepulses. To further investigate transitions after the initial activated state, we examined the temperature dependence of inactivation. The time constants of slow inactivation displayed little temperature and voltage dependence, but the degree of the inactivation increased substantially with increased temperature. Recovery from inac...Continue Reading

References

Apr 1, 1991·The Journal of Membrane Biology·P H Barry, J W Lynch
Jan 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·L H PhilipsonD F Steiner
Jan 1, 1990·Biophysical Journal·S C Lee, C Deutsch
Jan 1, 1990·The Journal of General Physiology·W N Zagotta, R W Aldrich
Apr 1, 1994·Biophysical Journal·E StefaniF Bezanilla
Feb 1, 1994·The Journal of General Physiology·T HoshiR W Aldrich
Feb 1, 1994·The Journal of General Physiology·W N ZagottaR W Aldrich
Feb 1, 1994·The Journal of General Physiology·W N ZagottaR W Aldrich
May 1, 1996·The Journal of General Physiology·P S SpectorM C Sanguinetti
Feb 1, 1996·Biophysical Journal·D I Levy, C Deutsch
Jan 1, 1997·European Biophysics Journal : EBJ·R Meyer, S H Heinemann

❮ Previous
Next ❯

Citations

Jan 18, 2006·Pflügers Archiv : European journal of physiology·Isabelle PlantePascal Daleau
Mar 8, 2005·Trends in Pharmacological Sciences·Eva DelpónJuan Tamargo
Sep 21, 2001·British Journal of Pharmacology·M LongobardoC Valenzuela
Oct 17, 2012·The Journal of General Physiology·Alain J LabroFrancisco Bezanilla
Sep 23, 2010·Journal of Molecular and Cellular Cardiology·Alvaro MacíasTeresa González
Dec 1, 2005·Progress in Biophysics and Molecular Biology·Harley T Kurata, David Fedida
Jul 21, 2001·Biophysical Journal·K G KlemicS W Jones
Dec 18, 2001·American Journal of Physiology. Cell Physiology·Thomas C RichDirk J Snyders

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.