Evidence for normal fibroblast cell membranes from individuals with Huntington's disease. A fluorescence probe study

Journal of the Neurological Sciences
C Sumbilla, J R Lakowicz

Abstract

We used fluorescence spectroscopy in an attempt to identify differences in the fibroblast cell membranes from individuals with Huntington's disease (HD), compared to those from normal individuals. Eight pairs of age- and sex-matched fibroblast cultures were examined, each using four different fluorescent probes. To maximize the possibility of finding a difference between the normal and HD cells fluorescence probes were selected which localize in different regions of the membrane. Also, these probes differed in their sensitivity to the surrounding environment. Procedures were developed for labeling of the fibroblasts while they were still attached to glass coverslips. All fluorescence measurements were done on attached cells, which were all found to be viable by staining with trypan blue. Both low (5-7) and high (11-13) passage cultures were examined since earlier reports indicated that normal and HD fibroblasts could be distinguished at low passage using a fluorescence method. Using these 16 cultures, and multiple determinations of the fluorescence emission spectra, anisotropies, and lifetimes, we found no significant difference between normal and HD fibroblasts. If such a difference exists, it appears to be too small for use a...Continue Reading

References

Mar 16, 1978·The New England Journal of Medicine·A LeonardiO Bugiani
May 1, 1977·Annals of Neurology·D S BarkleyJ H Menkes
Oct 1, 1979·Journal of Neurochemistry·J W PettegrewR M Stewart
Nov 1, 1979·Journal of Neurochemistry·P N Gray, S L Dana
Nov 1, 1979·Annals of Neurology·A B NoronhaB G Arnason
Feb 23, 1976·Biochemical and Biophysical Research Communications·S P HawkesM J Bissell
Apr 19, 1973·The New England Journal of Medicine·J H Menkes, N Stein
Oct 1, 1981·Journal of Neurochemistry·R M Mangano, R Schwarcz
Mar 1, 1982·Journal of the Neurological Sciences·R M Mangano, R Schwarcz
Jan 1, 1980·Journal of Biochemical and Biophysical Methods·J R Lakowicz
Jun 1, 1982·Journal of Neurochemistry·C Sumbilla, J R Lakowicz
Jul 20, 1982·Neuroscience Letters·G SarpelA Omachi
Aug 18, 1980·Life Sciences·D A ButterfieldW R Markesbery
Mar 9, 1981·Life Sciences·D A Butterfield, W R Markesbery
Jul 1, 1981·Journal of Neurology, Neurosurgery, and Psychiatry·T M DubbelmanG W Bruyn
May 1, 1982·Acta Neurologica Scandinavica·G C Beverstock, P L Pearson
Jul 1, 1980·Journal of the Neurological Sciences·A ZanellaG Scarlato
Jul 31, 1980·Biochemical and Biophysical Research Communications·P N GrayJ Elkins
Aug 29, 1980·Biochemical and Biophysical Research Communications·W A BialasD A Butterfield
Jan 1, 1980·Progress in Neuro-psychopharmacology·M Diez-EwaldJ V Gonzalez
Aug 1, 1981·Journal of Neurology, Neurosurgery, and Psychiatry·G C Beverstock, P L Pearson
Sep 1, 1981·Journal of Biochemical and Biophysical Methods·J R LakowiczA Balter

Citations

Jun 22, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Analía MariniBeatriz Farruggia
Oct 27, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Amir HandelmanGil Rosenman
Feb 14, 2014·Journal of the American Chemical Society·Ala'a O El-BallouliOmar F Mohammed
Jan 5, 1991·Journal of Molecular Biology·W Bujalowski, T M Lohman
Apr 1, 1989·Experimental Eye Research·M Larsen, L B Johansson
Feb 1, 1987·Analytical Biochemistry·J R LakowiczI Gryczynski
Jul 16, 1986·Biochemical and Biophysical Research Communications·R E HirschR L Nagel
Mar 30, 2004·Proteins·Mazdak KhajehpourJane M Vanderkooi
May 18, 2004·Proteins·Ernest L MaynardJeremy M Berg
Jan 1, 1986·Membrane Biochemistry·A S VerkmanJ A Dix
May 30, 2015·The Journal of Physical Chemistry. B·Subhajit Ghosh, Joykrishna Dey
May 13, 2015·The Journal of Physical Chemistry. B·Monika DzieciuchMariusz Kepczynski
Jan 8, 1999·Journal of Colloid and Interface Science·A Tsortos, G H Nancollas
Jan 1, 1997·Folding & Design·G T Dolphin, L Baltzer
Feb 27, 2001·Biophysical Journal·J M CaaveiroJ M González-Mañas
May 11, 1992·FEBS Letters·M KooijmanR van Grondelle
Nov 21, 2014·Inorganic Chemistry·Christoph KreitnerKatja Heinze
Jan 1, 1992·Biophysical Chemistry·D M GakamskyN V Shcherbatska
Feb 12, 2009·Journal of the American Chemical Society·Sasanka DekaLiberato Manna
May 10, 2012·The Journal of Physical Chemistry. B·Mousumi BanerjeeSamita Basu
Jul 19, 2014·The Journal of Organic Chemistry·Emmanuel E Nekongo, Vladimir V Popik
Jan 31, 2012·Environmental Science & Technology·John K EatonJonathan E Kenny
Nov 15, 2011·The Journal of Physical Chemistry. a·Thomas M CooperAlbert Fratini
Apr 8, 2011·The Journal of Physical Chemistry. a·Denis A IvanovMichael V Alfimov
Aug 4, 2011·Journal of the American Chemical Society·Jason R CoxTimothy M Swager
Jun 19, 2012·Chemical Reviews·Yuming YangFuyou Li
May 11, 2011·Analytical Chemistry·Russell Connally
Oct 21, 2011·Journal of the American Chemical Society·Melissa C Martínez-RiveraCecilia Tommos
May 18, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Maria LundinEva Blomberg
Oct 15, 2011·Analytical Chemistry·Peter C ThomasSamuel P Forry
Apr 9, 2014·The Journal of Physical Chemistry. B·Deepnath BajaniJoykrishna Dey
Jun 9, 2009·Journal of the American Chemical Society·Shinya TsukijiItaru Hamachi
Nov 29, 2012·The Journal of Physical Chemistry. B·Michael A FowlerErnesto Rivera
May 19, 2012·The Journal of Physical Chemistry. B·Adriana Gelover-SantiagoErnesto Rivera
Apr 9, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·Y W Yang, C C Teng
Dec 12, 1997·European Journal of Biochemistry·K KanaoriA Y Nosaka
Jul 1, 1997·Photochemistry and Photobiology·H SchneckenburgerR Steiner
Oct 1, 1996·Photochemistry and Photobiology·J R LakowiczZ Gryczynski
Jun 1, 1994·European Journal of Biochemistry·A Pineda-LucenaG Giménez-Gallego
May 1, 1994·European Journal of Biochemistry·T Coelho-SampaioA Vieyra
May 1, 1994·Biotechnology and Bioengineering·S B BambotG Rao
Jan 15, 1994·European Journal of Biochemistry·W BoeyB Hambly
Oct 1, 1995·Photochemistry and Photobiology·D L TraulF Sieber
May 1, 1994·Photochemistry and Photobiology·L UmaY Sharma
Jan 1, 1994·Photochemistry and Photobiology·J R LakowiczM L Johnson
Jan 15, 1993·European Journal of Biochemistry·R LeendersA J Visser
Jun 15, 1993·European Journal of Biochemistry·G SolainiG B Strambini
Nov 1, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Arpan BhattacharyaTushar Kanti Mukherjee
Jun 15, 1992·European Journal of Biochemistry·A P StassenR N Konings
Dec 15, 1992·European Journal of Biochemistry·J LeeA Bacher
Oct 1, 1991·European Journal of Biochemistry·W JiskootD J Crommelin
Sep 1, 1991·Photochemistry and Photobiology·G Ge, S Georghiou
Aug 1, 1991·Photochemistry and Photobiology·G Ge, S Georghiou
Jan 1, 2012·Chemical Science·Miao-Ping ChienNathan C Gianneschi
Jan 27, 2017·The Journal of Physical Chemistry. B·Hirak ChakrabortyAmitabha Chattopadhyay
Jan 1, 1990·Bioconjugate Chemistry·G E Means, R E Feeney
Dec 1, 1993·Photochemistry and Photobiology·G M AnsteadJ A Katzenellenbogen
Aug 1, 1993·Photochemistry and Photobiology·C SeidelK O Greulich
Dec 15, 1988·European Journal of Biochemistry·Z WasylewskiW Stryjewski
Jun 1, 1989·Journal of Neurochemistry·W G WoodF Schroeder
Dec 1, 1989·Photochemistry and Photobiology·N E Geacintov, H C Brenner
Sep 1, 1989·Photochemistry and Photobiology·C M RaoP B Rao
Apr 1, 1987·Photochemistry and Photobiology·N E Geacintov
Mar 1, 1987·Photochemistry and Photobiology·O Valdes-AguileraI E Kochevar
Jan 1, 1987·Annals of the New York Academy of Sciences·R A HarrisF R Simon
Nov 26, 2002·Biochemical and Biophysical Research Communications·Bidisa Sengupta, Pradeep K Sengupta
Oct 1, 1986·Journal of Bioenergetics and Biomembranes·G Lenaz, R Fato
Feb 16, 2005·Journal of Fluorescence·N A NemkovichA N Sobchuk
Nov 30, 2018·Journal of Biomedical Optics·Longhui LiShaoqun Zeng
Feb 1, 1987·Journal of Dairy Science·H M FarrellJ A Enyeart
Dec 12, 2018·Toxicological Sciences : an Official Journal of the Society of Toxicology·Lining ZhaoRutao Liu
Jun 1, 1992·The American Journal of Physiology·B R Takens-KwakA C Van Ginneken

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